Macrowine 2021
IVES 9 IVES Conference Series 9 Occurrence of methyl salicylate in lugana wines: aroma impact and biogenesis 

Occurrence of methyl salicylate in lugana wines: aroma impact and biogenesis 

Abstract

AIM: Methyl salicylate (MeSA) has been reported as a potentially impactful compound in Verdicchio wines produced in central Italy. Lugana is another white wine produced in the north-east of Italy from a grape locally known as Trebbiano di Soave, sharing a very similar genetic background with Verdicchio. The aims of this study were evaluating MeSA occurrence in Lugana, assessing its aroma impact on white wines aroma and elucidating its biogenesis during vinification.

METHODS: Fifteen Lugana wines were analysed for methyl salycilate content in comparison with Verdicchio, Pinot grigio and Garganega wines. MeSA impact on white wine aroma was studied by means of triangular test, adding MeSA at different concentrations. Possible routes of MeSA formation by yeast were investigated by means of a high throughput assay in which S. cerevisiae cells were put in contact with precursor such as salicylic acid (esterification) or glycosidic extracts (glycosidase). Sub-fractions of Lugana glycosidic extracts were also obtained by HPLC fractionation, allowing further evaluation of precursors role. MeSA formation was also followed during fermentation of Lugana must as well as during wine aging. All analyses of MeSA were carried out by SPME-GC-MS.

RESULTS: MeSA concentration in Lugana wines varied in the range 5-120 g/L, and was on average higher that in the other wines analysed. Sensory data showed that 20 µg/L of MeSA were sufficient to impact wine aroma, conferring floral and balsamic notes. Formation of MeSA was observed when yeast cells were in the presence of glycosidic extract, whereas esterification of salicylic acid was not confirmed. Release of MeSA from different HPLC fractions was observed, suggesting multiple possible precursors

CONCLUSIONS:

MeSA is present in Lugana wines at concentrations sufficiently high to impact wine aroma. MeSA odor in wine MeSA appears to be associated to floral attributes. MeSA formation is mostly due to yeast cleavage of grape glycosidic precursor

ACKNOWLEDGMENTS:

Biolaffort is acknowledged for financial support.

DOI:

Publication date: September 24, 2021

Issue: Macrowine 2021

Type: Article

Authors

Davide Slaghenaufi, Carla INDORATO, Eduardo VELA, Filippo FORTE, Giovanni LUZZINI, Maurizio UGLIANO,

Department of Biotechnology, University of Verona, Italy, 

Contact the author

Keywords

methyl salicylate; lugana; biogenesis; volatile compounds

Citation

Related articles…

Understanding vine response to Mediterranean summer stress for the development of adaptation strategies – in the kaolin case

In this video recording of the IVES science meeting 2023, Sara Bernardo (CITAB, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal) speaks about understanding vine response to Mediterranean summer stress for the development of adaptation strategies – in the kaolin case. This presentation is based on an original article accessible for free on OENO One.

Heatwaves impacts on grapevine physiology, berry chemistry & wine quality

Climate change impacts on both yields and quality have increased over the past decades, with the effects of extreme climate events having the most dramatic and obvious impacts. Increasing length and intensity of heatwaves associated with increased water stress necessitates a reevaluation of climate change responses of grapevine and, ultimately, a reconsideration of vineyard management practices under future conditions. Here we summarize results from a three-year field trial manipulating irrigation prior to and during heatwave events to assess impacts of water application rates on vine health and physiology, berry chemistry, and wine quality. We also highlight potential mitigation strategies for extreme heat, both in terms of water application, as well as other cultural practices that could be widely applicable.

Towards more coherent rules for alcohol labelling in the European Union

In its 2020 beating cancer plan, the european commission announced plans for mandatory warning signs for alcoholic beverages. However, no concrete legislative proposal has been put forward so far. Instead, ireland passed national legislation in 2023 that requires warning signs for all alcoholic beverages from 2026. Despite significant effects for the common market, the eu commission did not this challenge this law in the so-called tris notification procedure. We argue that the commission’s inaction is consistent with the case law of the european court of justice: in the absence of harmonized rules, member states have a large margin of discretion to enact national health measures.

Rootstock influence on xylem embolized vulnerability and scion behavior under severe water deficit

Severe water stress events can induce cavitation damage by xylem embolism in grapevine, diminishing plant hydraulic conductance. This work aimed to determine the rootstock effects on 1) xylem embolism vulnerability to understand its function failure under severe drought, including segmentation processes from leaf to root; and 2) hydraulic conductance across water deficit and its recovery. For this purpose, two complementary experiments were performed in one-year-old Vitis vinifera cv. Tempranillo grafted onto two different rootstocks (110-Richter and SO4) under well-watered 12L pot conditions. In experiment 1, the water-stress induced xylem embolism was monitored in leaves and stems, above and below grafting-point, by using “Cavicam” for determining the percentage of embolized vessels (at P12, P50 and P88).

Genetic study of wild grapevines in La Rioja region

Since the mid-1980s, several surveys have been carried out in La Rioja to search for populations of the sylvestris grapevine subspecies (Vitis vinifera L. subsp. sylvestris Gmelin). The banks of the Ebro River and its tributaries (Alhama, Cidacos, Leza, Iregua, Najerilla, Oja and Tirón rivers), as well as the surrounding vegetation of their valleys have been covered. So far, all the populations found are alluvial, forming part of the riparian vegetation of the Najerilla (the first reported population in La Rioja [1]), Iregua, and the vicinity of Oja valleys.