terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Effect of drought on grapevine wood fungal pathogen communities using a metatranscriptomics approach

Effect of drought on grapevine wood fungal pathogen communities using a metatranscriptomics approach

Abstract

Crops are facing increasing biotic and abiotic stress pressures due to global changes. However, trade-off mechanisms between these stresses and the underlying physiological processes are still poorly understood, especially in perennial crop species. To better understand these trade-offs, we studied the effect of drought on grapevine (Vitis vinifera) physiology and esca-related wood fungal communities. Esca is a vascular disease caused by a community of wood-infecting pathogenic fungi, and characterized by trunk necrosis, leaf scorch symptoms, yield losses, and mortality. This grapevine disease lead to xylem hydraulic failure and leaf symptoms are inhibited by severe drought. To characterize the molecular processes underlying the interactions between drought and esca, we conducted two experiments on 30-year-old Sauvignon blanc vines, expressing or not esca leaf symptoms, and subjected or not to drought stress under controlled conditions. Sapwood samples from the trunks were used to perform community-level transcriptomics analyses. Results will be also analyzed in the light of others metabolomics and ecophysiological data acquired on wood and leaf samples. Such an integrative approach will provide new insights into the understanding of grapevine/esca pathosystem under drought conditions, in terms of physiological and functional responses in either host and pathogens.

Acknowledgements: The authors thanks Université de Bordeaux for funding the GPR (Great Research Project) Bordeaux Plant Science.

DOI:

Publication date: October 6, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Marie Chambard1,2, Ninon Dell’Acqua1, Giovanni Bortolami1, Dario Cantù3, Nathalie Ferrer1, Gregory A. Gambetta4, Marie Foulongne-Oriol2, Chloé E. L. Delmas1

1 INRAE Bordeaux Nouvelle Aquitaine, UMR 1065 SAVE, 71 avenue Edouard Bourlaux – CS 20032, 33882 Villenave d’Ornon cedex
2 INRAE Bordeaux Nouvelle Aquitaine, UR 1264 MycSA, 71 avenue Edouard Bourlaux – CS 20032, 33882 Villenave d’Ornon cedex

3 Department of Viticulture and Enology, University of California, Davis, One Shields Ave, Davis, CA 95618, USA
4 INRAE Bordeaux Nouvelle Aquitaine, UMR 1287 EGFV, 71 avenue Edouard Bourlaux – CS 20032, 33882 Villenave d’Ornon cedex

Contact the author*

Keywords

Vitis vinifera Sauvignon Blanc, esca disease, drought, metatranscriptomics, physiology

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Vertical cordon training system enhances yield and delays ripening in cv. Maturana Blanca

The growing interest in minority grape varieties is due to their potential for adaptation to global warming and their oenological capabilities. However, the cultivation of these varieties has often been limited due to their low economic efficiency. One such example is Maturana Blanca, a recently recovered and authorized minority grape variety in the DOCa Rioja region, known for its remarkable oenological potential but low productivity. This study aimed to increase the yield of Maturana Blanca by implementing the vertical cordon training system, which allowed for a higher number of buds per plant and an increased cluster count per vine.

Organic mulches slightly influence wine phenolic composition and sensorial properties

Grapevines have traditionally been grown in semi-arid areas, but viticulture is now compromised by climate change. Therefore, it is necessary to implement environmentally friendly viticulture practices to adapt grapevines to current climatic conditions. In this context, organic mulches offer many benefits, such as reduced soil erosion and increased organic matter, soil water content and crop productivity. However, these practices must not compromise grape and wine quality. Therefore, the objective of this study was to evaluate the effect on wine physicochemical and phenolic composition and sensorial properties of different soil management practices on the vine row. Over four years, five soil treatments were examined in two different vineyards.

Discovering the process of noble rot: fungal ecology of grape berries during the noble rot transformation in different vineyards of the Tokaj wine region

Botrytis cinerea, a well-known grapevine pathogen, has more than 1200 host plants causing grey rot in grapevine berries. However, it can also result in a desirable phenomenon called noble rot under specific microclimate conditions. An extraordinary demonstration of this natural process can be observed in the creation of aszú wines within Hungary’s Tokaj wine region. Beside B. cinerea other fungi and yeasts are involved in the secondary metabolic development of the grape berry which contributes to the sensory and analytical characterization of noble rot wines.

Toasting and grain effect on Tempranillo red wine aged in Quercus petraea barrels

The barrel-making process is widely recognized as a crucial practice that affects the composition of barrel-aged wine. After the drying process, the staves are considered ready for barrel assembly, which includes the processes of bending and toasting the barrel structure. Toasting is considered one of the most critical stages in determining the physical and chemical composition of the staves, which can influence the chemical and sensory composition of the wine aged in barrels made from them [1].

Preliminary study of extraction of polysaccharides from pomace by high powered ultrasonic combined with enzymes

Red grape pomace can be an important source of polysaccharides, but currently they are little studied and even less with viable and environmental extraction processes (green extraction). These green techniques must be able to break the cell wall so that the compounds contained in the cells, including polysaccharides, are released and can have a great influence on extraction yields, the chemical structure of polysaccharides and applications in wines. Amongst the emerging green techniques most applied to the extraction of bioactive compounds, such as polysaccharides, high-power ultrasound (US) and enzyme-assisted extraction stand out.