Mining microbiome data to identify antagonists of grapevine downy mildew (Plasmopara viticola)

Aging on lees (AOL) has been used for wine aging for a long time, thanks to its ability to modify wine composition, improving sensory characteristics and stability. However, the prolonged contact with fermentation lees may increase the risk of developing sensory defects, due to the growth of unwanted microorganisms. Furthermore, AOL requires a large amount of work to manage bâtonnage and for topping up the barrels, significantly increasing production costs.

In the past, the contribution of non-Saccharomyces yeasts in winemaking has always been considered negative for their limited enological attitude if compared with Saccharomyces cerevisiae. In recent decades there has been a reevaluation of the role of non-Saccharomyces wine yeasts especially when used in combination and in support with S. cerevisiae (mixed fermentation). In this regard, selected non-Saccharomyces yeasts could be profitable used to give distinctive features, to enhance flavor and aroma complexity and to reduce the ethanol content of wines. Further emerging trends in the use of these yeasts are related to their role as bioprotectants and producers of health promoters compounds.

During alcoholic fermentation, nitrogen is an essential nutrient for yeast as it plays a key role in sugar transport and biosynthesis of wine aromatic compounds (thiols, esters, higher alcohols). The main issue of a lack in yeast assimilable nitrogen (yan) in winemaking is sluggish or stuck fermentations promoting the growth of alteration species which may lead to economic losses. However, correcting this nitrogen deficiency is sometimes not enough to restore proper fermentation performance. This suggests the existence of other nutritional shortages.

Viral diseases are a significant cause of both decreased grape quality and vineyard production. Important agents include grapevine leafroll-associated virus (glravs) and grapevine rupestris stem pitting-associated virus (grspav). However, conducting phytosanitary analysis of vineyards for viruses on-site is challenging, and molecular testing is generally expensive.

In the bottom of Val d’Illasi (Verona province), one of the major valleys which passes through the Lessini mountains, viticulture is widely extended. In the territory belonging to Illasi and Tregnago villages, which includes ca. 1100 ha of vineyards, devoted to produce Soave and Valpolicella DOC wines, an experimental survey was conducted on a network of twenty five reference vineyards.