GiESCO 2019 banner
IVES 9 IVES Conference Series 9 Open-GPB 9 Category: Open-GPB-2024 ( Page 15 )

Proceedings of the Open International Conference on Grapevine Physiology and Biotechnology 2024

Discover the abstracts and posters presented during the Open International Conference on Grapevine Physiology and Biotechnology 2024 (Open-GPB2024), held in Logroño, La Rioja, Spain, from July 7th to 11th, 2024. This conference is organised by the Instituto de Ciencias de la Vid y del Vino (ICVV).

Oral presentations by sessions

Flash presentations by sessions

Posters by sessions

Search a document

OPEN GPB 2024 – View all

IVES Conference SeriesOpen-GPBOpen-GPB-2024Poster - Plant vegetative and reproductive development

Phenotypical impact of a floral somatic mutation in the cultivar Listán Prieto

The accession Criolla Chica Nº2 (CCN2) is catalogued as a floral mutation of cultivar Criolla Chica (synonym for cv. Listán Prieto). Contrary to what is observed in hermaphrodite-cultivated varieties like Criolla Chica, CCN2 exhibits a prevalence of masculinized flowers. Aiming to study the incidence and phenotypical implications of this mutation, CCN2 plants were deeply studied using Criolla Chica ‘Ballista’ (CCBA) as control plants. For each CCN2 plant, two inflorescences per shoot were sampled and segmented into proximal, mid and distal positions, relative to the pedicel. Flowers were observed through magnifying lens and classified according to OIV151 descriptor.

View article

IVES Conference SeriesOpen-GPBOpen-GPB-2024Poster - Advances in precision viticulture and phenotyping

Physical-mechanical berry skin traits as powerful indicators of resistance to botrytis bunch rot

The ongoing climate change results in increasing mean air temperature, which is manifested by weather extremes or sudden changes between drought and local heavy rainfalls. These changing conditions are especially challenging for the established grapevine varieties growing under cool climate conditions due to an increased biotic infection pressure. Thus, the scope of most grapevine breeding programs is the selection of mildew fungus-resistant and climatic adapted grapevines with balanced, healthy yield and outstanding wine quality. Since no resistances or candidate genes have yet been described for Botrytis bunch rot (BBR), physical-mechanical traits like berry size and thick, impermeable berry cuticles phenotyped with high-throughput sensors represent novel effective parameters to predict BBR.

View article

IVES Conference SeriesOpen-GPBOpen-GPB-2024Poster - Biotic interactions

Physiological means to curb the enthusiasm of viruses from infecting grapevines  

The two most deadly viruses infecting and threatening the productivity of grapevines worldwide are leafroll and red blotch viruses. There is no cure for viral diseases other than roguing the symptomatic vines and replacing them with certified vines derived from clean, virus-tested stocks.
Given that phloem plays a central role in virus infection, this study aimed to purge the virus by girdling the phloem of leafroll-infected vines at different phenological stages of infected grapevines. Phloem-girdling was performed on canes at veraison to varying regions between the proximal and distal clusters.

View article

IVES Conference SeriesOpen-GPBOpen-GPB-2024Poster - Abiotic interactions

PIWIs’ variation in drought response under semi-controlled conditions 

Grapevine interspecific hybrids (PIWIs, from German “pilzwiderstandsfähige Rebsorten” meaning fungus tolerant grapevine cultivars), offer a promising alternative to traditional cultivars in many wine regions due to their tolerance to certain fungal diseases. This makes them a potential solution for sustainable wine production, particularly under organic systems. Despite extensive research on certain agronomic traits and susceptibility to biotic diseases, such as powdery and downy mildews, the response of these cultivars to abiotic stressors, such as drought, remains unclear. Our study aims to investigate the eco-physiological traits of two commercial PIWI cultivars, Muscaris and Souvignier gris, at the leaf level to evaluate their response to drought stress.

View article

IVES Conference SeriesOpen-GPBOpen-GPB-2024Poster - New biotechnological tools

Plant regeneration via somatic embryogenesis and preliminary trials for the application of the DNA-free genome editing in grapevine cv. Corvina veronese

Grapevine (Vitis spp.) is a globally significant fruit crop, and enhancing its agronomic and oenological traits is crucial to meet changing agricultural conditions and consumer demands. Conventional breeding has played a key role in domesticating grapevine varieties, but it is a time-consuming process to develop new cultivars with desirable traits for cultivation.
New plant breeding techniques (NpBTs) offer a potential revolution in grapevine cultivation, and genome editing has shown promise for targeted mutagenesis. The success of these biotechnological approaches relies on efficient in vitro regeneration protocols, particularly through somatic embryogenesis (SE).

View article

IVES Conference SeriesOpen-GPBOpen-GPB-2024Poster - Abiotic interactions

Postharvest ozone treatment in grapevine white cultivars: Effects on grape volatile composition

During postharvest management, the metabolism of fruits remains active and continuous physico-chemical changes occur. Ozone treatment has an elicitor effect on secondary metabolites and the treatment conditions can influence the grape response to the stress (Bellincontro et al., 2017; Botondi et al., 2015). Regarding volatile organic compounds (VOCs), previous studies showed that ozone treatment during postharvest dehydration induces the biosynthesis of terpenes in Moscato bianco grapes (Río Segade et al., 2017). It is well known that grape VOCs greatly influence the organoleptic properties of wines, particularly terpenes in aromatic varieties.

View article

IVES Conference SeriesOpen-GPBOpen-GPB-2024Poster - New biotechnological tools

Preliminary steps of a protocol to isolate transcription factors bound to a specific DNA locus in grapevine using CRISPR-dCas9 system

Cis-acting regulatory elements are DNA sequences that can be bound by transcription factors to regulate the expression of genes in a condition-dependent and tissue-specific way. It is nowadays possible to search for DNA motives and sequences that a given transcription factor is binding or at least can, but it is still hard to have a glance at all the transcription factors that are contemporaneously located at the same locus. Inspired by an existing technique that uses the CRISPR-Cas system in mammal cells, we are trying to develop a protocol to study such regulation in Vitis vinifera. Using the highly sequence-specific binding capacity of a catalytically inactive Cas9 protein (dCas9), our idea is to set up a system to target a desired sequence and precipitate all the crosslinked proteins and distantly interacting chromatin at this locus and analyze them.

View article

IVES Conference SeriesOpen-GPBOpen-GPB-2024Poster - Advances in precision viticulture and phenotyping

Protection of genetic diversity: maintenance and developements of a grapevine genebank in Hungary

Among the items preserved in gene banks, the old standard and autochthonous varieties represent an increasing value, since these varieties may have properties to make their cultivation more effective under changing climatic conditions. The increasingly extreme weather is a huge challenge for the viticulture. Collectional varieties can also play important role in protection against pests and pathogens. A genebank ensures not only the preservation of rare varieties, but also gives the opportunity for more knowledge and research of these varieties.

View article

IVES Conference SeriesOpen-GPBOpen-GPB-2024Poster - Advances in omics and big data

Proteomic profiling of grape berry presenting early loss of mesocarp cell vitality

From fruit set to ripening, the grape berry mesocarp experiences a wide range of dynamic physical, physiological, and biochemical changes, such as mesocarp cell death (MCD) and hydraulic isolation. The premature occurrence of such events is a characteristic of the Niagara Rosada (NR) variety, utilised as table grapes and winemaking. In our opinion, the onset of ripening would not cause MCD, but a down-regulation of respiratory enzymes during the early loss of cell viability, while maintaining membrane integrity. For this, we investigated three distinct developmental stages (green (E-L33), veraison (E-L35), and ripe (E-L39)) of NR berries by label-free proteomics, enzymatic respiratory activity and outer mesocarp imaging. Cell wall-modifying proteins were found to accumulate differently throughout ripening, while cytoplasmic membranes continue intact.

View article

IVES Conference SeriesOpen-GPBOpen-GPB-2024Poster - Abiotic interactions

Radiation-associated effects on regulated deficit irrigation management in grapevine cv. Cabernet Sauvignon

The main challenge of regulated deficit irrigation (RDI) research is to isolate the factors that come with RDI, the direct effect of plant water status from the indirect ones like increased radiation and temperature changes on the cluster zone. This study aims to isolate the effects of vine water status from the effects of increased radiation on the phenolic composition of grapes subjected to RDI.
A three-year study on an RDI experiment where radiation was controlled was implemented in a commercial vineyard of Cabernet Sauvignon in Chile. Four RDI treatments based on partial evapotranspiration (ET) irrigation were established. Irrigation treatments were 100% ET, 70% ET, 50-100% ET (50% ET before veraison and 100% ET afterward), and 35-100% ET (35% ET before veraison and 100% ET afterward).

View article