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IVES 9 IVES Conference Series 9 Open-GPB 9 Category: Open-GPB-2024 ( Page 8 )

Proceedings of the Open International Conference on Grapevine Physiology and Biotechnology 2024

Discover the abstracts and posters presented during the Open International Conference on Grapevine Physiology and Biotechnology 2024 (Open-GPB2024), held in Logroño, La Rioja, Spain, from July 7th to 11th, 2024. This conference is organised by the Instituto de Ciencias de la Vid y del Vino (ICVV).

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OPEN GPB 2024 – View all

IVES Conference SeriesOpen-GPBOpen-GPB-2024Poster - Biotic interactions

Exploring the inner secrets of grapevine: a journey through plant-microbe interactions

Throughout centuries of anthropocentric breeding, plants have been selectively bred to enhance their quality traits and yield, often overlooking the importance of neglected attributes, like those involved in the interactions with beneficial microorganisms. This phenomenon led to an alteration in the distribution of photosynthetic products, shifting from defence mechanisms to growth, commonly described as 'domestication syndrome'. Addressing the losses stemming from this condition is imperative just as unravelling the concealed communication between grapevines and beneficial microorganisms.

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IVES Conference SeriesOpen-GPBOpen-GPB-2024Poster - Abiotic interactions

Exploring the regulatory role of the grapevine MIXTA homologue in cuticle formation and abiotic stress resilience

The outer waxy layer of plant aerial structures, known as the cuticle, represents an important trait that can be targeted to increase plant tolerance against abiotic stresses exacerbated by environmental transition. The MIXTA transcription factor, member of the R2R3-MYB family, is known to affect conical shape of petal epidermal cells in Anthirrinum, cuticular thickness in tomato fruit and trichome formation and morphology in several crops. The aim of this study was to investigate the role of the grapevine MIXTA homologue by phenotypic and molecular characterization of overexpressing and knock-out grapevine lines.

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IVES Conference SeriesOpen-GPBOpen-GPB-2024Poster - Advances in precision viticulture and phenotyping

Exploring zoxamide sensitivity in Plasmopara viticola populations: implications for fungicide management in precision agriculture

Fungicides play a critical role in managing grapevine downy mildew caused by the oomycete Plasmopara viticola, a biotrophic and polycyclic pathogen with a high risk of fungicide resistance. Zoxamide, categorized as a low to medium resistance risk, disrupts cell division by inhibiting tubulin polymerization. Resistance to zoxamide is uncommon in field isolates. This six-year study (2017-2022) aimed to detect and quantify zoxamide sensitivity in P. viticola populations across varying resistance pressures in Italian grapevine regions. Analysis of 126 samples from 57 vineyards, mainly in North-Eastern Italy, revealed that most samples exhibited EC50, EC95, and MIC values below 0.1 and 10 mg/L of zoxamide, respectively. Nineteen vineyards showed reduced sensitivity (MIC>100 mg/L), but only four samples were characterized by 24-54% resistant oospores at >100 mg/L of zoxamide.

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IVES Conference SeriesOpen-GPBOpen-GPB-2024Poster - Table grapes, raisins and postharvest physiology

Fertilization Lysimeters provide new insights into the needs and impacts of N nutrition on table grape performance and fruit yield and quality

Table grape production requires adequate nitrogen (N) supply to sustain vine performance and obtain high yields. However, excess agricultural N fertilization is a major source of groundwater contamination and air pollution. Therefore, there is a strong need for empirically based precision N fertilization schemes in vineyards, for optimizing grape yield and quality while minimizing their environmental impact.
Our aim was to unequivocally quantify table grape N requirements, elucidate the drivers of daily N uptake, and quantify the relationship between fertigation N levels and vine growth, fruit yield, composition, and quality. For this, forty 'Early Sweet' (early-maturing, white) and 'Crimson seedless' (late-maturing, red) vines were grown in 500L drainage-lysimeters for 2 fruiting seasons, while subjected to five continuous N fertigation treatments ranging from 10 to 200 ppm.

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IVES Conference SeriesOpen-GPBOpen-GPB-2024Poster - Plant vegetative and reproductive development

Fertilization with Seaweed (Laminaria japonica) on the characteristics of the bunch and the quality of the grape must of ‘Cabernet Sauvignon’

The objective of the present work was to study the influence of the foliar application of seaweed (Laminaria japonica), on the bunch and on the must in the ‘Cabernet Sauvignon’ grape. The experiment was carried out in the years 2021/2022, in a 21-year-old commercial vineyard, in the municipality of “Dom Pedrito” – “Rio Grande do Sul” (RS). A completely randomized experimental design was used, with 4 treatments and 4 replications (7 plants per replication). The treatments were: T1- control treatment; T2- Exal Powder 5 g L-1; T3- Hidro Exal 15 ml L-1; T4- Exal Powder 5 g L-1+ Hidro Exal 15 ml L-1.

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IVES Conference SeriesOpen-GPBOpen-GPB-2024Poster - Abiotic interactions

Freeze-thaw temperature oscillations promote increased differential gene expression during grapevine bud dormancy

In northern cold climate conditions, chilling requirement fulfillment in dormant grapevine buds is slowed or stopped by subzero temperatures impacting the transcriptional processes needed to complete chilling requirement. Cabernet Franc and Reisling in Geneva, NY were used to determine the impact of natural oscillating temperatures on grapevine bud transcriptional activity during light and dark periods of a two-week period in January with fluctuating diurnal winter temperatures. Cabernet Franc and Reisling bud samples were collected at 32 time points during the natural vineyard temperature cycle at 6:00 (dark), 14:00 (light) and 18:00 (dark) hours) to monitor gene expression in consecutive freezing and non-freezing temperature oscillations. Genotype, light and dark, and temperature oscillations conditions were explored.

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Flash - Abiotic interactionsIVES Conference SeriesOpen-GPBOpen-GPB-2024

From genes to vineyards: system biology and new breeding technologies for water stress tolerance in grapevines

One of the major challenges for food security and sovereignty is to produce stress-tolerant plants without introducing foreign DNA, because the legislative process, that bans transgenics, challenges us to find new solutions for producing plants that can survive the drought. To achieve this goal, we need to identify genes that can be modified to improve stress tolerance in plants. In this work, we present an online tool for exploring the transcriptome of grapevines under water stress, which is one of the most important abiotic stresses affecting viticulture. The tool is based on a comprehensive collection of rna-seq data from 997 experiments, covering four different tissues (leaf, root, berry, and shoot), various levels of water stress, and diverse genetic backgrounds (cultivars and rootstocks) with different levels of tolerance to water stress.

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Opening and closing lectures

From grapevines to extreme environments … and back?

I performed my PhD in grapevine physiology under the supervision of Dr. H. Medrano, standing in the vineyards from pre-dawn to sunrise during many hot, wet and sunny days with my colleagues J.M.E. and J.B. I also spent many days and nights facing ticks year-round working in Mediterranean macchias with J.Gu. and M.M. Later I was able to supervise PhD students on grapevines – like A.P. and M.T. – and on Mediterranean vegetation – like J.Gal. With the incorporation to the group of M.R.-C. ‘the puzzle’ was completed and, combining the aforementioned studies, we could conclude (more than 20 years ago) things like: (1) stomatal conductance is the best proxy for ‘water stress’ in studies on photosynthesis; (2) steady-state chlorophyll fluorescence retrieves photosynthesis under saturating light; (3) photoinhibition is not a major photosynthetic limitation under water stress; (4) mesophyll conductance instead is; and (5) mesophyll conductance is a major driver of leaf water use efficiency.

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IVES Conference SeriesOpen-GPBOpen-GPB-2024Oral - New biotechnological tools

From protein-centered to gene-centered approaches to investigate DNA-protein interactions in grapevine

DNA-binding proteins play a pivotal role in critical cellular processes such as DNA replication, transcription, recombination, repair, and other essential activities. Consequently, investigating the interactions between DNA and proteins is of paramount importance to gain insights into these fundamental cellular mechanisms. Several methodologies have been devised to uncover DNA-protein interactions, which can be broadly categorized into two approaches. The “protein-centered” approach focuses on identifying the DNA sequences bound by a specific transcription factor or a set of TFs. Techniques falling within this category include chromatin immunoprecipitation, and protein-binding microarrays.

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IVES Conference SeriesOpen-GPBOpen-GPB-2024Oral - Abiotic interactions

From soil to canopy, the diversity of adaptation strategies  to abiotic constraints in grapevine

Climate change is here. One of the main consequences is an increase in the frequency and severity of abiotic stresses which mostly occur in a combined manner. Grapevine, which grows in a large diversity of pedo-climatic conditions, has presumably evolved different mechanisms to allow this widespread adaptation. Harnessing the genetic diversity in these mechanisms will be central to the future of viticulture in many traditional wine growing areas. The interactions between the scion and the rootstock through grafting add an additional level of diversity and adaptive potential to explore.
At the physiological level, these mechanisms are related to processes such as root system development and functioning (water and nutrient uptake), interactions with the soil microbiome, gas exchange regulation, hydraulic properties along the soil-plant-atmosphere continuum, reserve storage, short and long distance signaling mechanisms and plasticity for some of these traits.

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