Effect of post-harvest ozone treatment on secondary metabolites biosynthesis and accumulation in grapes and wine

This work aimed to evaluate the evolution of phenolic compounds during white winemaking process up to bottling and 12 months storage, together with the influence of different antioxidant strategies

This communication was estracted from a study concerning the viticultural characterization of A.V.A. “Friuli-Grave” area sponsored by Chamber of Commerce of Pordenone.

The objective of the present work is to assess yeast effects on the development of wine varietal aroma throughout aging and on wine longevity.

Three independent experiments were carried out; two fermenting semi-synthetic musts fortified with polyphenols and aroma precursors extracted from Tempranillo (1) or Albariño (2) grapes and with synthetic precursors of polyfunctional mercaptans (PFMs), and a third in which a must, mixture of 6 different grape varieties was used. In all cases, fermentations were carried out by different Saccharomyces cerevisiae strains and one S. kudriavzevii, and the obtained wines were further submitted to anoxic accelerated aging to reproduce bottle aging. The volatile profile of the wines was analyzed using several chromatographic procedures, in order to provide a comprehensive evaluation of wine aroma. Aroma compounds analyzed included fermentation volatile metabolites, grape-derived aroma compounds including PFMs, and Strecker aldehydes (SA).

Results revealed that the effects of yeast on wine aroma throughout its self-life extend along three main axes:

1. A direct or indirect action on primary varietal aroma and on its evolution during wine
aging.

2. The direct production of SA during fermentation and/or their delayed formation by producing the required reagents (amino acids + dicarbonyls) for Strecker degradation
during anoxic aging.

3. Producing acids (leucidic, branched acids) precursors to fruity esters. More specifically, and leaving aside the infrequent de novo formation, the action of the different strains of yeast on primary varietal aroma takes four different forms:

1.- Speeding the hydrolysis of aroma precursors, which leads to early aroma formation without changing the amount of aroma formed. In the case of labile molecules, such as linalool, the enhancement of young wine aroma implies a short-living wine. 2.- Metabolizing the aroma precursor, reducing the amounts of aroma formed, which can be of advantage for negative aroma compounds, such as TDN or guaiacol; 3.- Transforming grape components into aroma precursors, increasing the amounts of aroma formed, as for ethyl cinnamate, leucidic acid or vinylphenols; 4.- Forming reactive species such as vinylphenols able to destroy varietal polyfunctional mercaptans.

Overall, it can be concluded that the yeast carrying alcoholic fermentation not only influences fermentative wine aroma but also affects to the wine varietal aroma, to its evolution during aging and to the development of oxidative off-odors

Un paysage viticole est une relation entre des formes, dimension objective, et la perception que nous en avons, dimension subjective, émotionnelle. La viticulture n’est pas seulement productrice d’un vin, elle contribue également à façonner le paysage. Pourtant, jusqu’à présent, la connaissance des terroirs était principalement basée sur la caractérisation de leur aptitude à produire des vins de qualité.

AIM: Strains belonging to M. pulcherrima/fructicola clade are frequently isolated from flowers, fruits and grape musts, and exhibit a broad spectrum of enzymatic activities and antimicrobial potential (Morata et al., 2019; Sipiczki, 2020; Vicente et al. 2020).