IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Skin And Seed Extracts Differently Behave Towards Salivary Proteins

Skin And Seed Extracts Differently Behave Towards Salivary Proteins

Abstract

Background: Polyphenols extracted from skins and seeds showed different sensory attributes including astringency and bitterness. In previous studies, it has been demonstrated that extracts obtained either from skins or seeds interact differently with salivary proteins. Red grape winemaking consists of a maceration of the whole berries in which both skins and seeds are mixed together; however, no information on the mutual influence that skins and seeds could have on the reactivity towards saliva of hydroalcoholic extracts is known. In this study, five different  wine model solutions were prepared: the first one contained only skins (Sk), the second one contained only seeds (Sd) and the remaining three contained different skin/seed ratios, as detailed below: A (ratio 2:1 skin : seed), B(ratio 1:1 skin : seed) and C (ratio 1:2 skin: seed).

Methods: HPLC analyses were performed to determine the content of total native anthocyanins, acetaldehyde and polymeric pigments. Iron reactive phenolics, BSA reactive tannins (BSArT), vanillin reactive flavans (VRF) were also determined. The potential astringency of red samples was evaluated in vitro by the Saliva Precipitation index (SPI), which measures the reactivity of salivary proteins towards wine polyphenols.

Results: The results obtained in this experiment highlighted important differences in the behavior of the samples as a function of the different skin:seed ratio. When skins and seeds  were simultaneously present (samples A, B and C), a significant lower content of anthocyanins with respect to Sk was observed. This was likely due to a possible adsorption of pigments on cell walls contained in pomaces. As the amount  of seeds increased in the solutions containing also skins, the content of VRF, BSArT, PP and acetaldehyde linearly increased. After 24 months of aging under controlled conditions, all the trends observed at 0 time were confirmed and appeared to be enhanced. Concerning the interactions toward salivary proteins, as expected, sample Sd showed the highest SPI. Surprisingly, when skins were added to a model solution containing seeds, a decrease of SPI occurred, although VRF and BSArT increased. This suggests that  anthocyanins and polymeric pigments in A, B and C samples determined a lower reactivity of compounds contained in the whole solution towards saliva proteins. SPI values are not correlated to the amount of VRF and BSArT in the samples.

Conclusion:

Results of our study highlighted not only the important role of the skin:seed ratio in regulating the extraction of compounds from berries, but also that of anthocyanins extracted from skins in decreasing the reactivity of grape compounds towards saliva.

DOI:

Publication date: June 24, 2022

Issue: IVAS 2022

Type: Poster

Authors

Errichiello Francesco1, Guerriero Antonio1, Picariello Luigi1, Coppola Francesca1, Rinaldi Alessandra1,2, Forino Martino1 and Gambuti Angelita1

1Department of Agricultural Sciences, Grape and Wine Science Division, University of Naples Federico II
2Biolaffort, 126 Quai de la Souys, 33100 Bordeaux, France

Contact the author

Keywords

skin/seed extract; anthocyanins; polymeric pigments; astringency.

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Aromatic maturity is a cornerstone of terroir expression in red wine

In this video recording of the IVES science meeting 2023, Stéphanie Marchand (University of Bordeaux, ISVV, INRAE, UMR 1366 OENOLOGIE, Villenave d’Ornon, France) speaks about the aromatic maturity as a cornerstone of terroir expression in red wine. This presentation is based on an original article accessible for free on OENO One.

Wine racking in the winery and the use of inerting gases

The O2 uptake in the different winemaking processes is generally considered to be negative for the sensory characteristics of white and rosé wines. Wine racking is a critical point of O2 uptake, as the large surface area of the wine exposed during this operation and the inability to maintain an effective inert gas blanket over it.
The objective was to study O2 uptake during the racking of a model wine without using inert gases and to compare it with the purging of the destination tank with different inert gases.

The effect of wine matrix on the initial release of volatile compounds and their evolution in the headspace

There is evidence in the literature that non-volatile wine matrix can modify the release and therefore the perception of the compounds involved in wine aroma [1-3].

The interplay between water deficit and nitrogen and potassium nutrition in Vitis vinifera L.

Climate change is expected to provoke an increase in the frequency and intensity of drought events and water scarcity that will have detrimental effects on photosynthesis and plant yield. To sustain an appropriate plant yield under sub-optimal conditions, a common practice is the application of high amounts of fertilizers with negative environmental consequences. The present study aims at evaluating the interplay between water and nutrient availability, namely nitrogen (N) and potassium (K), in two grapevine cultivars with a different sensitivity to water shortage stress. Two-year-old Vitis Vinifera cv. Cabernet Sauvignon and Grenache grapevine plants grafted on SO4 rootstock have been transferred in pots under semi-environmental conditions.

Determination of Aroma Compounds in Grape Mash under Conditions of Tasting by On-line Near-Infrared Spectroscopy

The production of high-quality wines requires the use of high-quality grapes. Some compounds originating from grapes may negatively influence the odour and flavour of the resulting wine in their original form or as precursors for off-odours and –flavours. Therefore, a rapid evaluation of the grapes directly upon receival at the winery is advantageous. Up to now, grape aroma is mainly evaluated by tasting, however, this leads to subjective results. The use of near-infrared (NIR) spectroscopy allows a rapid, objective and destruction-free analysis without previous sample preparation. Moreover, the measurement can be integrated into an existing process without additional sampling.

IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Skin And Seed Extracts Differently Behave Towards Salivary Proteins

Skin And Seed Extracts Differently Behave Towards Salivary Proteins

Abstract

Content of the article

References

Section for all references

DOI:

Publication date: June 24, 2022

Issue: IVAS 2022

Type: typeofthepublication

Authors

author1, author2, author3

Presenting author

Description

List of affiliations ¹ ² ³

Contact the author

Email address (with mailto: link)

Keywords

List of different keywords (keyword1, keyword2, keyword3)

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Agronomic and oenological characterization of the intraspecific cross ‘Passau’ in the aim of its commercial use

The study of new wine grape cultivars can be interesting to diversify the local wine productions without using international varieties. With this aim some Vitis vinifera intraspecific crosses obtained by Prof. Dalmasso in the 1930s and registered in the Italian National Catalogue in 1977, have been studied in the last years.

Intravarietal diversity: an opportunity for climate change adaptation

Merlot grapevine is the second wine cultivar most planted in the world and especially in the Bordeaux wine region. This cultivar has many advantages in producing high quality wine; however, in the last decade, climate change has increased the sugar concentration in berries at harvest and shortened the maturation cycle. If this has been up to now a great opportunity to improve wine quality profile, we are touching the tipping point. High sugar concentration at harvest induces high alcool content in wine which can negatively impact wine quality. There are many viticultural and oenological practices possible to limit this effect. In this study we focus on plant material through intra-varietal diversity of Merlot cultivar.

Anti/prooxidant activity of wine polyphenols in reactions of adrenaline auto-oxidation

Adrenaline (epinephrine) belongs to catecholamine class. It is a neurotransmitter and both a hormone which is released by the sympathetic nervous system and adrenal medulla in response to a range of stresses in order to regulate blood pressure, cardiac stimulation, relaxation of smooth muscles and other physiological processes. Adrenaline exhibits an effective antioxidant capacity (1). However, adrenalin is capable to auto-oxidation and in this case it generates toxic reactive oxygen intermediates and adrenochrome. Under in vitro conditions, auto-oxidation of adrenaline occurs in an alkaline medium (2).

Characterization of intact glycoside aroma precursors of recovered minority Spanish red grape varieties by High-Resolution Mass Spectrometry

In Spain, the wide diversity of red grapevine varieties represents an advantage when choosing the most suitable one for cultivation based on different climatic conditions, without implying a loss of their enological potential.

Elicitors application in two maturation stages of Vitis vinifera L. cv Monastrell: changes on the skin cell walls

AIM: In a recent study, it was determined that the mid-ripening period is the most suitable for the application of methyl jasmonate (MeJ), benzothiadiazole BTH and MeJ+BTH on Monastrell grapes, to favor maximum accumulation of phenolic compounds at the time of harvest. However, the increase in the anthocyanin content of