IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Skin And Seed Extracts Differently Behave Towards Salivary Proteins

Skin And Seed Extracts Differently Behave Towards Salivary Proteins

Abstract

Background: Polyphenols extracted from skins and seeds showed different sensory attributes including astringency and bitterness. In previous studies, it has been demonstrated that extracts obtained either from skins or seeds interact differently with salivary proteins. Red grape winemaking consists of a maceration of the whole berries in which both skins and seeds are mixed together; however, no information on the mutual influence that skins and seeds could have on the reactivity towards saliva of hydroalcoholic extracts is known. In this study, five different  wine model solutions were prepared: the first one contained only skins (Sk), the second one contained only seeds (Sd) and the remaining three contained different skin/seed ratios, as detailed below: A (ratio 2:1 skin : seed), B(ratio 1:1 skin : seed) and C (ratio 1:2 skin: seed).

Methods: HPLC analyses were performed to determine the content of total native anthocyanins, acetaldehyde and polymeric pigments. Iron reactive phenolics, BSA reactive tannins (BSArT), vanillin reactive flavans (VRF) were also determined. The potential astringency of red samples was evaluated in vitro by the Saliva Precipitation index (SPI), which measures the reactivity of salivary proteins towards wine polyphenols.

Results: The results obtained in this experiment highlighted important differences in the behavior of the samples as a function of the different skin:seed ratio. When skins and seeds  were simultaneously present (samples A, B and C), a significant lower content of anthocyanins with respect to Sk was observed. This was likely due to a possible adsorption of pigments on cell walls contained in pomaces. As the amount  of seeds increased in the solutions containing also skins, the content of VRF, BSArT, PP and acetaldehyde linearly increased. After 24 months of aging under controlled conditions, all the trends observed at 0 time were confirmed and appeared to be enhanced. Concerning the interactions toward salivary proteins, as expected, sample Sd showed the highest SPI. Surprisingly, when skins were added to a model solution containing seeds, a decrease of SPI occurred, although VRF and BSArT increased. This suggests that  anthocyanins and polymeric pigments in A, B and C samples determined a lower reactivity of compounds contained in the whole solution towards saliva proteins. SPI values are not correlated to the amount of VRF and BSArT in the samples.

Conclusion:

Results of our study highlighted not only the important role of the skin:seed ratio in regulating the extraction of compounds from berries, but also that of anthocyanins extracted from skins in decreasing the reactivity of grape compounds towards saliva.

DOI:

Publication date: June 24, 2022

Issue: IVAS 2022

Type: Poster

Authors

Errichiello Francesco1, Guerriero Antonio1, Picariello Luigi1, Coppola Francesca1, Rinaldi Alessandra1,2, Forino Martino1 and Gambuti Angelita1

1Department of Agricultural Sciences, Grape and Wine Science Division, University of Naples Federico II
2Biolaffort, 126 Quai de la Souys, 33100 Bordeaux, France

Contact the author

Keywords

skin/seed extract; anthocyanins; polymeric pigments; astringency.

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Drought responses in Chardonnay and Sauvignon blanc grapevine cultivars: Mechanistic insights and varietal contrasts

This study explored the responses of Chardonnay and Sauvignon blanc grapevine cultivars to water deficit across four years, uncovering their shared patterns and distinctive coping mechanisms. The research was conducted in a commercial vineyard located in Isla de Maipo, Chile. Various characterization approaches were employed including plant water potentials (), gas exchange measurements, shoot vulnerability curves, productivity assessments, and leaf cell water relations. Linear mixed models and sensitivity analyses were performed using various statistical methods to evaluate cultivar responses to water deficit. As the water deficit progressed, both cultivars displayed a parallel reduction in stomatal conductance, leaf turgor, and increased shoot embolism.

Innovative approaches for fungicide resistance monitoring in precision management of grapevine downy mildew

Effective control with fungicides is essential to protect grapevine from downy mildew, a devastating disease caused by the oomycete Plasmopara viticola. Managing this disease faces challenges in maintaining fungicide efficacy as the number of modes of action decreases and the risk of fungicide resistance increases. Long-term measures should address strains resistant to multiple modes of action, that can be selected by the repeated use of single-site fungicides. For these reasons, a precision management of the disease, that considers the selection of the best fungicide schedule according to the sensitivity profile of the pathogen population, is needed.

Vegetative dose heights ‘Cabernet Sauvignon’ and its influence on fruit and wine quality

The leaf area is of fundamental importance so that the plant can realize adequate levels of photosynthesis for the accumulation of reserves and to reach a suitable maturation of
the berries. In this sense, the objective was to evaluate the effect of different lengths of the stalks from the first support wire, in the must and in the wine of ‘Cabernet Sauvignon’.

Influence of a spontaneous cover crop on the vineyard and soil erosion under Mediterranean climate

Sixty five % of the agricultural area of the Basque Country located in the DO Ca Rioja corresponds to vineyards. More than 40% of it has an average slope greater than 10%, which makes it sensitive to erosive processes. Furthermore, it is foreseeable that extreme weather events (storms, hail, extreme heat and cold, etc.) will be favored due to climate change. Cover cropping can mitigate this risk, and therefore the objective of this work is to evaluate the impact that a vegetable cover has on the agronomic behavior of the vineyard, the quality of the grape and soil erosion. For this, a trial has been carried out with a Graciano variety vineyard with a slope between 10% -20% during the years 2020 and 2021. Conventional tillage management in the area has been compared (4-6 passes per year of tillage machinery) versus spontaneous vegetation cover management in the vineyard. This implies not tilling and allowing the grass of the land to colonize the range between the lines of vines, controlling their height through 1-3 mowing passes per year, always trying to affect the surface of the land as little as possible. The vegetative growth, yield and quality of the grape and wine was measured. Furthermore, erosion has been measured using Gerlasch boxes. The yield was lower in the second year of the trial in the cover crop treatment, but erosion was significantly reduced.

Characterizing chemical influences of smoke on wine via novel application of 13c-labelled smoke

Smoke impact is an ongoing and growing issue for vintners across the globe, with the west coast of the U.S. and Australia being two of the largest wine industries impacted. Wine has shown to be especially sensitive to smoke exposure, often acquiring off-flavor sensory characteristics, such as “burnt rubber”, “ashy”, or other medicinal off-flavors.1 While several studies have examined the chemical composition of smoke influences on wine, some studies disagree on what compounds are having the largest impact on smell and flavor.2 This study is designed as a bottom-up approach to inventory the chemical compounds derived from smoke from a grassland-like fire that are potentially influencing wine chemical composition.

IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Skin And Seed Extracts Differently Behave Towards Salivary Proteins

Skin And Seed Extracts Differently Behave Towards Salivary Proteins

Abstract

Content of the article

References

Section for all references

DOI:

Publication date: June 24, 2022

Issue: IVAS 2022

Type: typeofthepublication

Authors

author1, author2, author3

Presenting author

Description

List of affiliations ¹ ² ³

Contact the author

Email address (with mailto: link)

Keywords

List of different keywords (keyword1, keyword2, keyword3)

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

A DNA-free editing approach to help viticulture sustainability: dual editing of DMR6-1 and DMR6-2 enhances resistance to downy mildew 

The sustainability of viticulture hinges on maintaining quality and yield while reducing pesticide use. Promising strides in this direction involve the development of clones with enhanced disease tolerance, particularly through the knockout of plant susceptibility genes. Knocking out of Downy Mildew Resistant 6 (DMR6) led to increased levels of endogenous salicylic acid (SA), a regulator of immunity, resulting in enhanced tolerance to Downy Mildew (DM) and other diseases in various crops.

OENOLOGICAL TANNINS FOR PREVENTING THE LIGHT-STRUCK TASTE IN WHITE AND ROSÉ WINES

The light exposure of wine can be detrimental as a relevant loss of aromas takes place [1] and light-induced reactions can occur. The latter involves riboflavin (RF), a photosensitive compound, that is fully reduced by acquiring two electrons. When the electron-donor is methionine, the light-struck taste (LST) can appear leading to cooked cabbage, onion and garlic odours-like [2]. The use of oenological tannins can limit the appearance of LST in both model wine [3] and white wine [4]. This research aimed to evaluate the impact of certain oenological tannins, selected in a previous study as the most effective against LST [5], in both white and rosé wines.

Physiological responses of crimson seedless (Vitis vinifera) grapevines to altered micro climatic conditions and different water treatments in the Breede River Valley of South Africa

Challenging conditions created by limited water supply and changes in the climate require an understanding of the physiological status of table grapes along the whole value chain. This is critical to develop tools for regulatory management of growth balances and grape quality. This study aimed to determine the impact of different amounts of water and an altered micro-climate (complete covering of vineyards with plastic) on the physiological reaction of the grapevine during the growth season.

Phenolic composition and chromatic characteristics of blends of cv. Tempranillo wines from vines grown with different viticultural techniques in a semi-arid area

The quality and color stability of red wines are directly related to content and distribution of phenolic compounds. However, the climate change produces the asynchrony between the dates of technological and maturity of grapes. The crop-forcing technique (CF) restores the coupling between phenolic and technological ripeness while limits vineyard yields. Blending of wines is frequently used to equilibriate composition of wines and to increase their stability, color and quality. The aim of the present work is to study the phenolic composition and color of wine blends made with FW (wines from vines subjected to CF) and CW (wines for vines under the usual cultivation practices).

Vers la maîtrise de l’effeuillage pré-floral de la vigne

Dans le cadre de TerclimPro 2025, Thibaut Verdenal a présenté l’article IVES Technical Reviews. Retrouvez la présentation ci-dessous ainsi que l’article associé : https://ives-technicalreviews.eu/article/view/8405