WAC 2022 banner
IVES 9 IVES Conference Series 9 WAC 9 WAC 2022 9 4 - WAC - Posters 9 Red wine extract and resveratrol from grapevines could counteract AMD by inhibiting angiogenesis promoted by VEGF pathway in human retinal cells

Red wine extract and resveratrol from grapevines could counteract AMD by inhibiting angiogenesis promoted by VEGF pathway in human retinal cells

Abstract

Age-related macular degeneration (AMD) that is the main cause of visual impairment and blindness in Europe which is characterized by damages in the central part of the retina, the macula. This degenerative disease of the retina is mainly due to the molecular mechanism involving the production and secretion of vascular endothelial growth factor (VEF). Despite therapeutic advances thanks to the use of anti-VEGF, the progression of the disease is often observed without reverse vision quality. New therapies have emerged such as surgical pharmacological and special attention has been paid to prevention, where diet plays a preponderant role. Indeed, antioxidant such as resveratrol, a polyphenol of grapevines, can prevent VEGF secretion induced by stress from retinal cells. Resveratrol can not only reduce oxidative stress but also alter cellular and molecular signaling as well as physiological effects involved in ocular diseases such as AMD. In this context, we investigate the potential effect of red wine extract (RWE) on the secretion and its signaling pathway in human retinal cells ARPE-19. In order to investigate the effect of RWE in ARPE-19, a quantitative and qualitative analysis of the RWE was performed by HPLC MS/MS. We highlight that RWE are able to decreased whether the protein expression and the secretion of VEGF-A from ARPE-19 in a concentration-dependent manner. This alteration of VEGF-A production is associated with a decreased of VEGF-receptor2 (VEGF-R2) protein expression and its phosphorylated intracytoplasmic domain. Afterwards, kinase pathway activation is disturbing and RWE prevents the phosphorylation of MEK and ERK 1/2 in human retinal cells ARPE-19. According to our results, polyphenolic cocktails could present a potential interest in a prevention strategy against AMD.

Acknowledgments:

This work was supported by grants from the ANRT N°°2016/0003, by a French Government grant managed by the French National Research Agency under the program “Investissements d’Avenir”, reference ANR-11-LABX-0021, the Conseil Régional Bourgogne, Franche-Comte (PARI grant) and the FEDER (European Funding for Regional Economic Development), the “Bureau Interprofessionnel des Vins de Bourgogne” (BIVB), and by the Bordeaux Metabolome Facility and MetaboHUB (ANR-11-INBS-0010) project

DOI:

Publication date: June 27, 2022

Issue: WAC 2022

Type: Article

Authors

Clarisse CORNEBISE, Flavie Courtaut, Marie Taillandier-Coindard, Josep Valls-Fonayet, France, Tristan Richard, David Monchaud, Virginie Aires, Dominique Delmas

Presenting author

Clarisse CORNEBISE – Université de Bourgogne Franche-Comté, Dijon, F-21000, France ; INSERM Research Center U1231 – Cancer and Adaptive Immune Response Team, Dijon, Bioactive Molecules and Health research group, F-21000, France

Université de Bourgogne Franche-Comté, Dijon, F-21000, France ; INSERM Research Center U1231 – Cancer and Adaptive Immune Response Team, Dijon, Bioactive Molecules and Health research group, F-21000, France, Université de Bourgogne Franche-Comté, Dijon, F-21000, France ; INSERM Research Center U1231 – Cancer and Adaptive Immune Response Team, Dijon, Bioactive Molecules and Health research group, F-21000, France, Unité de Recherche Oenologie, EA 4577, USC 1366 INRA-ISVV, F-33882 Villenave d’Ornon, France, Unité de Recherche Oenologie, EA 4577, USC 1366 INRA-ISVV, F-33882 Villenave d’Ornon, France, Université de Bourgogne Franche-Comté, F-21000 Dijon, France; Institut de Chimie Moléculaire (ICMUB), CNRS UMR6302, UBFC, F-21078 Dijon, France, Université de Bourgogne Franche-Comté, Dijon, F-21000, France ; INSERM Research Center U1231 – Cancer and Adaptive Immune Response Team, Dijon, Bioactive Molecules and Health research group, F-21000, France, Université de Bourgogne Franche-Comté, Dijon, F-21000, France ; INSERM; Centre Anticancéreux Georges François Leclerc, F-21000 Dijon, France; Research Center U1231 – Cancer and Adaptive Immune Response Team, Dijon, Bioactive Molecules and Health research group, F-21000, France

Contact the author

Keywords

Polyphenols, red wine extract, AMD, retinal cells, ARPE-19, degenerative diseases, ocular diseases

Tags

IVES Conference Series | WAC 2022

Citation

Related articles…

Mobile device to induce heat-stress on grapevine berries

Studying heat stress response of grapevine berries in the field often relies on weather conditions during the growing season. We constructed a mobile heating device, able to induce controlled heat stress on grapes in vineyards. The heater consisted of six 150 W infrared lamps mounted in a profile frame. Heating power of the lamps could be controlled individually by a control unit consisting of a single board computer and six temperature sensors to reach a pre-set temperature. The heat energy applied to individual berries within a cluster decreases by the squared distance to the heat source, enabling the establishment of temperature profiles within individual clusters. These profiles can be measured by infrared thermography once a steady state has been reached. Radiant flux density received by a berry depending on the distance was calculated based on a view factor and measured lamp surface temperature and resulted to 665 Wm-2 at 7cm. Infrared thermography of the fruit surface was in good agreement with measurements conducted with a thermocouple inserted at epidermis level. In combination with infrared thermography, the presented device offers possibilities for a wide range of applications like phenotyping for heat tolerance in the field to proceed in the understanding of the complex response of plants to heat stress. Sunburn necrosis symptoms were artificially induced with the aid of the device for cv. Bacchus and cv. Sylvaner in the 2020 and 2021 growing season. Threshold temperatures for sunburn induction (LT5030min) were derived from temperature data of single berries and visual sunburn assessment, applying logistic regression. A comparison of threshold temperatures for the occurrence of sunburn necrosis confirmed the higher susceptibility of cv. Bacchus. The lower susceptibility of cv. Sylvaner did not seem to be related to its phenolic composition, rendering a thermoprotective role of berry phenolic compounds unlikely.

Effect of vigour and number of clusters on eonological parameters and metabolic profile of Cabernet Sauvignon red wines

Vegetative growth and yield are reported to affect grape and wine quality. They can be controlled through different techniques linked to vine management. The objective of this research was to determine the effect of vine vigour and number of clusters per vine on physicochemical composition and phenolic profile of red wines. The experiment was carried out during two vegetative cycles, with cv. Cabernet Sauvignon grafted onto Paulsen 1103. Three vine vigour were defined, according to shoot weight at previous harvests, being low, medium and high. Five treatments of number of clusters were used for each vigour, with 15, 22, 29, 36, and 45 clusters per vine. Grapes from all treatments were harvested in the same day from Brix and total acidity criteria. Thirty days after bottling, classical analyzes and phenolic compounds were performed. As results, different responses were obtained from each vintage. In 2020, a dry season from veraison to harvest, grapes and wines obtained from low vigour treatment and 45 clusters per vine was the highest in sugar and alcohol content respectively, while grapes and wines from high vigour and 15 clusters presented the lowest sugar and alcohol content. Total anthocyanins were higher in treatment with low vigour and 15 clusters, while the lowest amounts were found in low vigour with 45 clusters, as well as medium and high vigour with 36 clusters per vine. Total tannins were higher in high vigour with 22 clusters and medium vigour with 29 clusters, while were lower in low vigour with 36 clusters. In 2021, a wet season at harvest, responses were different, and great variations were observed between treatments. As conclusions, yield and vine vigour had strong influence on grape and wine quality, promoting different enological potentials on which can be indicated/used for aging strategies of red and even rosé wines.

Assessing the climate change vulnerability of European winegrowing regions by combining exposure, sensitivity and adaptive capacity indicators

Winegrowing regions recognized as protected designations of origin (PDOs) are closely tied to well defined geographic locations with a specific set of pedoclimatic attributes and strictly regulated by legal specifications. However, climate change is increasingly threatening these regions by changing local conditions and altering winegrowing processes. The vulnerability to these changes is largely heterogenous across different winegrowing regions because it is determined by individual characteristics of each region, including the capacity to adapt to new climatic conditions and the sensitivity to climate change, which depend not only on natural, but also socioeconomic and legal factors. Accurate vulnerability assessments therefore need to combine information about adaptive capacity and climate change sensitivity with projected exposure to new climatic conditions. However, most existing studies focus on specific impacts neglecting important interactions between the different factors that determine climate change vulnerability. Here, we present the first comprehensive vulnerability assessment of European wine PDOs that spatially combines multiple indicators of adaptive capacity and climate change sensitivity with high-resolution climate projections. We found that the climate change vulnerability of PDO areas largely depends on the complex interactions between physical and socioeconomic factors. Homogenous topographic conditions and a narrow varietal spectrum increase climate change vulnerability, while the skills and education of farmers, together with a good economic situation, decrease their vulnerability. Assessments of climate change consequences therefore need to consider multiple variables as well as their interrelations to provide a comprehensive understanding of the expected impacts of climate change on European PDOs. Our results provide the first vulnerability assessment for European winegrowing regions at high spatiotemporal resolution that includes multiple factors related to climate exposure, sensitivity, and adaptive capacity on the level of single winegrowing regions. They will therefore help to identify hot spots of climate change vulnerability among European PDOs and efficiently direct adaptation strategies.

Mapping and tracking canopy size with VitiCanopy

Understanding vineyard variability to target management strategies, apply inputs efficiently and deliver consistent grape quality to the winery is essential. However, despite inherent vineyard variability, the majority are managed as if they are uniform. VitiCanopy is a simple, grower-friendly tool for precision/digital viticulture that allows users to collect and interpret objective spatial information about vineyard performance. After four years of field and market research, an upgraded VitiCanopy has been created to achieve a more streamlined, technology-assisted vine monitoring tool that provides users with a set of superior new features, which could significantly improve the way users monitor their grapevines. These new features include:
• New user interface
• User authentication
• Batch analysis of multiple images
• Ease the learning curve through enhanced help features
• Reporting via the creation of colour maps that will allow users to assess the spatial differences in canopies within a vineyard.
Use-case examples are presented to demonstrate the quantification and mapping of vineyard variability through objective canopy measurements, ground-truthing of remotely sensed measurements, monitoring of crop conditions, implementation of disease and water management decisions as well as creating a history of each site to forecast quality. This intelligent tool allows users to manage grapevines and make informed management choices to achieve the desired production targets and remain profitable.

Influence of weather and climatic conditions on the viticultural production in Croatia

The research includes an analysis of the impact of weather conditions on phenological development of the vine and grape quality, through monitoring of four experimental cultivars (Chardonnay, Graševina, Merlot and Plavac mali) over two production years. In each experimental vineyard, which were evenly distributed throughout the regions of Slavonia and The Croatian Danube, Croatian Uplands,