terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Use of UHPH to improve the implantation of non-Saccharomyces yeasts

Use of UHPH to improve the implantation of non-Saccharomyces yeasts

Abstract

Ultra High-Pressure Homogenization (UHPH) is a high-pressure pumping at 300 MPa (>200 MPa) with a subsequent depressurization against a highly resistant valve made of tungsten carbide covered by ceramic materials or carbon nanoparticles. The intense impact and shear efforts produce the nano-fragmentation of colloidal biopolymers including the elimination of microorganism (pasteurization or sterilization depending on in-valve temperature) and the inactivation of enzymes. This technology is extremely gentle with molecules with sensory impact remaining unaffected compounds as terpenes, thiols, and anthocyanins, and protected of ulterior oxidations by the inactivation of oxidative enzymes (PPOs). The use of UHPH in must before fermentation is a powerful technology to eliminate wild microorganism and to facilitate the implantation of non-Saccharomyces inoculated as starters. In this work we show the efficient implantation of several weak-fermenter non-Saccharomyces and the effect on the release of volatile thiols.

Acknowledgements: This research was funded by MICIN, project PID2021-124250OB-I00.

References: 

1)  Morata, A. et al. (2020) Front. Nutr.7, 598286. https://doi.org/10.3389/fnut.2020.598286  

2)  Vaquero, C. et al. (2022) Food Bioprocess Technol. 15, 620–634. https://doi.org/10.1007/s11947-022-02766-8  

3)  Loira, I. et al. (2018) Innov. Food. Sci. Emerg. Technol. 50, 50–56. https://doi.org/10.1016/j.ifset.2018.10.005   

4)  Bañuelos, M.A. et al. (2020) Food Chem. 332, 127417. https://doi.org/10.1016/j.foodchem.2020.127417

DOI:

Publication date: October 5, 2023

Issue: ICGWS 2023

Type: Article

Authors

Antonio MORATA1*, Iris LOIRA1, Juan Manuel DEL FRESNO1, Carlos ESCOTT1, Felipe PALOMERO1, Carmen LÓPEZ1, Buenaventura GUAMIS2, Mª Antonia BAÑUELOS3, Cristian VAQUERO1, Carmen GONZÁLEZ1

1enotecUPM, Dept. Chemistry and Food Technology, ETSIAAB, Universidad Politécnica de Madrid, 208040; Madrid, Spain
2YPSICON ADVANCED TECHNOLOGIES S.L, Via Trajana 50-56 Nave 21, 08020, Barcelona, Spain
3enotecUPM, Dept. Biotechnology, ETSIAAB, Universidad Politécnica de Madrid, 208040; Madrid, Spain

Contact the author*

Keywords

UHPH, Implantation, non-Saccharomyces, Lachancea thermotolerans, terpenes, thiols

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Uncovering the interplay between Copper and SO2 tolerance in Saccharomyces cerevisiae

[lwp_divi_breadcrumbs home_text="IVES" use_before_icon="on" before_icon="||divi||400" module_id="publication-ariane" _builder_version="4.20.4" _module_preset="default" module_text_align="center" module_font_size="16px" text_orientation="center"...

Analysis of volatile composition of interaction between the pathogen E. necator and two grapevine varieties

Volatile organic compounds (VOCs) are emitted by nearly all plant organs of the plants, including leaves. They play a key role in the communication with other organisms, therefore they are involved in plant defence against phytopathogens. In this study VOCs from grapevine leaves of two varieties of Vitis vinifera infected by Erysiphe necator were analysed. The varieties were selected based on their susceptibility to pathogen, Kishmish Vatkana has the Ren1 resistance gene and Zamarrica showed high susceptibility in previous trials.

A sensometabolomic approach to understand wine mouthfeel percepts

Targeted analytical methods can overlook compounds that are a priori unknown to play a role in the mouthfeel sensations. This limitation can be overcome with the information provided by untargeted metabolomic analysis using UPLC‐QTOF-MS. To this end, an untargeted metabolomic approach applied to 42 red wines has allowed development of a model with predictive capacity by cross-validation for the “dry”, “oily” and “unctuous” sensations perceived by a sensory panel. The optimal PLS model for “dry” retained compounds with positive regression coefficients (≥ 0.17) including a trimer procyanidin, a peptide, and four anthocyanins.

The generation of suspended cell wall material may limit the effect of ultrasound in some varieties

The disruptive effect exerted by high-power ultrasound (US) on plant cell walls, natural barriers to the diffusion of compounds of interest during the maceration of red wines, is established as the reason behind the chromatic improvement that its treatment causes. However, sometimes this improvement is not observed, especially with short maceration times. The presence of a high quantity of suspended cell wall material, which formation is favored by the sonication, could be the cause of this lack of positive results since this cell wall material has a high affinity for phenolic compounds.

Application of antagonistic Metschnikowia strains against Botrytis cinerea in vineyards 

Less and less chemical plant protection products are approved by the E U. Plant pathogenic fungi become increasingly resistant to the active ingredients that have been around for a long time. Besides, there is a valid demand for effective products that can be applied in organic cultivation.
We examined Metschnikowia strains under laboratory conditions in order to find effective strains against B. cinerea. The antimicrobial mechanism of these yeasts is based on the competition for the ferric ions from the environment. Metschnikowia cells release the pulcherriminic acid which chelates with Fe3+, forming the pigment pulcherrimin.