terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Use of UHPH to improve the implantation of non-Saccharomyces yeasts

Use of UHPH to improve the implantation of non-Saccharomyces yeasts

Abstract

Ultra High-Pressure Homogenization (UHPH) is a high-pressure pumping at 300 MPa (>200 MPa) with a subsequent depressurization against a highly resistant valve made of tungsten carbide covered by ceramic materials or carbon nanoparticles. The intense impact and shear efforts produce the nano-fragmentation of colloidal biopolymers including the elimination of microorganism (pasteurization or sterilization depending on in-valve temperature) and the inactivation of enzymes. This technology is extremely gentle with molecules with sensory impact remaining unaffected compounds as terpenes, thiols, and anthocyanins, and protected of ulterior oxidations by the inactivation of oxidative enzymes (PPOs). The use of UHPH in must before fermentation is a powerful technology to eliminate wild microorganism and to facilitate the implantation of non-Saccharomyces inoculated as starters. In this work we show the efficient implantation of several weak-fermenter non-Saccharomyces and the effect on the release of volatile thiols.

Acknowledgements: This research was funded by MICIN, project PID2021-124250OB-I00.

References: 

1)  Morata, A. et al. (2020) Front. Nutr.7, 598286. https://doi.org/10.3389/fnut.2020.598286  

2)  Vaquero, C. et al. (2022) Food Bioprocess Technol. 15, 620–634. https://doi.org/10.1007/s11947-022-02766-8  

3)  Loira, I. et al. (2018) Innov. Food. Sci. Emerg. Technol. 50, 50–56. https://doi.org/10.1016/j.ifset.2018.10.005   

4)  Bañuelos, M.A. et al. (2020) Food Chem. 332, 127417. https://doi.org/10.1016/j.foodchem.2020.127417

DOI:

Publication date: October 5, 2023

Issue: ICGWS 2023

Type: Article

Authors

Antonio MORATA1*, Iris LOIRA1, Juan Manuel DEL FRESNO1, Carlos ESCOTT1, Felipe PALOMERO1, Carmen LÓPEZ1, Buenaventura GUAMIS2, Mª Antonia BAÑUELOS3, Cristian VAQUERO1, Carmen GONZÁLEZ1

1enotecUPM, Dept. Chemistry and Food Technology, ETSIAAB, Universidad Politécnica de Madrid, 208040; Madrid, Spain
2YPSICON ADVANCED TECHNOLOGIES S.L, Via Trajana 50-56 Nave 21, 08020, Barcelona, Spain
3enotecUPM, Dept. Biotechnology, ETSIAAB, Universidad Politécnica de Madrid, 208040; Madrid, Spain

Contact the author*

Keywords

UHPH, Implantation, non-Saccharomyces, Lachancea thermotolerans, terpenes, thiols

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Overall conceptual characterization of aged dry white wines using a mental descriptive questionnaire

The purpose of the present study was to understand the overall concept of an aged dry white wine using a descriptive mental questionnaire. A total of 680 worldwide participants, grouped according to their involvement in the wine business, replied to an online questionnaire to characterize the sensory analytical and synthetic descriptors of an aged dry white wine. The descriptors were selected using a Check-All-That-Apply (CATA) approach concerning wine colour, aroma, taste, mouthfeel, and global appreciation.

Influence of p-Coumaric Acid and Micronutrients on Growth and 4-Ethylphenol Production by Brettanomyces bruxellensis

The wine spoilage caused by Brettanomyces bruxellensis is one of the global concerns for winemakers. Detecting the presence of B. bruxellensis using routine laboratory culture techniques becomes challenging when cells enter the viable but not culturable (VBNC) state. This study aims to investigate the impact of p-coumaric acid (a volatile phenol precursor) and micronutrients on B. bruxellensis’ culturability, viability, and volatile phenol production under sulfite stress. In red wine, exposure to a high sulfite dose (100.00 mg L-1 potassium metabisulfite) resulted in immediate cell death, followed by a recovery of culturability after two weeks.

Differences in metabolism among species and hybrids of the genus Saccharomyces during wine fermentation unveiled by multi-omic analysis 

Yeast species S. cerevisiae, S. uvarum, S. kudriavzevii and their hybrids present clear metabolic differences, even when we compared S. cerevisiae wine versus wild strain. These species and hybrids produced significantly higher amounts of glycerol, organic acids, 2,3-butanediol, and 2-phenyl ethanol and a reduction of the ethanol yield, properties very interesting in the sector to deal with climate change effects. To understand the existing differences, we have used several omics techniques to analyze the dynamics of the (intra- and extracellular) metabolomes and/or transcriptomes of representative strains of S. cerevisiae, S. uvarum, S. kudriavzevii, and hybrids.

A phylogenomic study reveals the major dissemination routes of ‘Tempranillo Tinto’ in the Iberian Peninsula

‘Tempranillo Tinto’ is a black-berried Iberian cultivar that originated from a hybridization between cvs. ‘Benedicto’ and ‘Albillo Mayor’ [1]. Today, it is the third most widely grown wine grape cultivar worldwide with more than 200,000 hectares of vineyards mostly distributed along the Iberian Peninsula, where it is also known as ‘Cencibel’, ‘Tinta de Toro’, ‘Tinta Roriz’, and ‘Aragonez’, among other synonyms. Here, we quantified the intra-varietal genomic diversity in this cultivar through the study of 35 clones or ancient vines from seven different Iberian wine-making regions. A comparative analysis after Illumina whole-genome sequencing revealed the presence of 1,120 clonal single nucleotide variants (SNVs).

Wine racking in the winery and the use of inerting gases

The O2 uptake in the different winemaking processes is generally considered to be negative for the sensory characteristics of white and rosé wines. Wine racking is a critical point of O2 uptake, as the large surface area of the wine exposed during this operation and the inability to maintain an effective inert gas blanket over it.
The objective was to study O2 uptake during the racking of a model wine without using inert gases and to compare it with the purging of the destination tank with different inert gases.