terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Use of UHPH to improve the implantation of non-Saccharomyces yeasts

Use of UHPH to improve the implantation of non-Saccharomyces yeasts

Abstract

Ultra High-Pressure Homogenization (UHPH) is a high-pressure pumping at 300 MPa (>200 MPa) with a subsequent depressurization against a highly resistant valve made of tungsten carbide covered by ceramic materials or carbon nanoparticles. The intense impact and shear efforts produce the nano-fragmentation of colloidal biopolymers including the elimination of microorganism (pasteurization or sterilization depending on in-valve temperature) and the inactivation of enzymes. This technology is extremely gentle with molecules with sensory impact remaining unaffected compounds as terpenes, thiols, and anthocyanins, and protected of ulterior oxidations by the inactivation of oxidative enzymes (PPOs). The use of UHPH in must before fermentation is a powerful technology to eliminate wild microorganism and to facilitate the implantation of non-Saccharomyces inoculated as starters. In this work we show the efficient implantation of several weak-fermenter non-Saccharomyces and the effect on the release of volatile thiols.

Acknowledgements: This research was funded by MICIN, project PID2021-124250OB-I00.

References: 

1)  Morata, A. et al. (2020) Front. Nutr.7, 598286. https://doi.org/10.3389/fnut.2020.598286  

2)  Vaquero, C. et al. (2022) Food Bioprocess Technol. 15, 620–634. https://doi.org/10.1007/s11947-022-02766-8  

3)  Loira, I. et al. (2018) Innov. Food. Sci. Emerg. Technol. 50, 50–56. https://doi.org/10.1016/j.ifset.2018.10.005   

4)  Bañuelos, M.A. et al. (2020) Food Chem. 332, 127417. https://doi.org/10.1016/j.foodchem.2020.127417

DOI:

Publication date: October 5, 2023

Issue: ICGWS 2023

Type: Article

Authors

Antonio MORATA1*, Iris LOIRA1, Juan Manuel DEL FRESNO1, Carlos ESCOTT1, Felipe PALOMERO1, Carmen LÓPEZ1, Buenaventura GUAMIS2, Mª Antonia BAÑUELOS3, Cristian VAQUERO1, Carmen GONZÁLEZ1

1enotecUPM, Dept. Chemistry and Food Technology, ETSIAAB, Universidad Politécnica de Madrid, 208040; Madrid, Spain
2YPSICON ADVANCED TECHNOLOGIES S.L, Via Trajana 50-56 Nave 21, 08020, Barcelona, Spain
3enotecUPM, Dept. Biotechnology, ETSIAAB, Universidad Politécnica de Madrid, 208040; Madrid, Spain

Contact the author*

Keywords

UHPH, Implantation, non-Saccharomyces, Lachancea thermotolerans, terpenes, thiols

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Lipids at the crossroads of protection: lipid signalling in grapevine defence mechanisms

Understanding grapevine molecular processes and the underlying defence responses is vital for developing sustainable disease control strategies. Lipid signalling pathways, involving the synthesis and degradation of lipid molecules, have emerged as a key regulator in plant defence against pathogens. This study aims to elucidate the role of fatty acids and lipid signalling in grapevine’s defence response to P. viticola infection. The expression of lipid metabolism-related as well as lipid signalling genes was analysed, by qPCR, in three grapevine genotypes: Chardonnay (susceptible), Regent (tolerant) with Rpv3-1 resistance loci, and Sauvignac (resistant) harbouring a pyramid of Rpv12 and Rpv3-1 resistance loci.

Role of anthocyanins and copigmentation in flavonol solubility in red wines 

Over the last years, due to climate change, several red wines, such as the Sangiovese wines, have been often subjected to loss of clarity due to the formation of deposits of fine needle-shaped crystals. This phenomenon turned out to be due to an excess of quercetin (Q) and its glycosides (Q-Gs) in wines. These compounds are synthesized to a large extent when grapes are excessively exposed to UVB radiations in vineyards[1]. Unfortunately, it is not easy to predict the degree of Q precipitation because its solubility strongly depends on the wine and matrix composition[2].

Symbiotic microorganisms application in vineyards: impacts on grapevine performance and microbiome

Microorganism-based inoculants have been suggested as a viable solution to mitigate the adverse effects of climate change on viticulture. However, the actual effectiveness of these inoculants when applied under field conditions remains a challenge, and their effects on the existing soil microbiota are still uncertain. This study investigates the impact of arbuscular mycorrhizal fungi inoculation on grapevine performance and microbiome. The study was conducted in a vineyard of Callet cultivar in Binissalem, Mallorca, Spain. Two different treatments were applied: control and inoculation with commercial mycorrhizae complex of Rhizoglomus irregulare applied to plants through irrigation.

Options to replace or reduce the sulphite content in Tannat red wines produced with minimal intervention

Several Uruguayan wineries have begun to produce wines with minimal intervention, to increase the sustainability of their vineyards and wines. These wines are characterized by the minimum intervention in the management of the vineyard, its harvest, vinification, conservation and aging1,2. Sulfur dioxide (SO2) is not used or is used in reduced doses, although chitosan can be substituted or supplemented1. The objective of this research is to evaluate SO2 reduction or replacement options adapted to the production of Tannat red wines with minimal intervention. Vinification of the Tannat grapes with autochthonous yeasts (LN) was carried out during the 2023 vintage.

Reconstructing ancient microbial fermentation genomes from the wine residues of Herod, Roman king of Judea

The fortress of the Herodium, built towards the end of the first century BCE/ante Cristo, on the orders of Herod the Great, Roman client king of Judea, attests the expansion of Roman influence in the eastern Mediterranean. During archaeological excavations of the Herodium in 2017[1], a winery was discovered on the ground floor of the palace, with an assortment of clay vessels in situ, including large dolia – clay fermentation vessels each capable of fermenting up to 300-400 L of wine. Thanks to the recent progresses in the field of paleogenomics[2], we could analyse the organic material consistent with grape pomace at the bottom of these vessels, by extracting and sequencing the DNA using shotgun metagenomics and targeted capture, aiming for enrichment of DNA from fermentation associated microbes.