terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Identification of several glycosidic aroma precursors in six varieties of winemaking grapes and assessment of their aroma potential by acid hydrolysis

Identification of several glycosidic aroma precursors in six varieties of winemaking grapes and assessment of their aroma potential by acid hydrolysis

Abstract

In winemaking grapes, it is known that most aroma compounds are present as non-volatile precursors, such as glycosidic precursors. In fact, there is strong evidence supporting the connection between the content of aroma precursors and the aromatic quality of wine [1]. Acid hydrolysis is preferred to reveal the aroma potential of winemaking grapes, as it predicts more accurately the chemical rearrangements occurring during fermentation in acidic environments [2]. In this study, a method involving a fast fermentation followed by acid hydrolysis at 75ºC was used to evaluate the accumulation of aroma compounds over time in fractions obtained from six different varieties of winemaking grapes. The aim was to identify the specific aroma glycosidic precursors responsible for this variability by confirming their structures and establishing correlations with the liberated aromas. The variability of aroma compounds with respect to both time and variety was investigated through a two-way ANOVA. A Principal Component Analysis of the volatile aromas confirmed the differences between grape varieties and hydrolysis time. The most notable differences were observed in the hydrolysates at 96 hours, where the accumulation of aromas such as norisoprenoids and phenols was prominent. A method involving Solid Phase Extraction followed by UHPLC-QTOF-MS/MS was employed to isolate and identify the pool of aroma precursors. Most of the glycosides identified based on their fragmentation patterns were indeed disaccharides with the structure of pentose-hexose and hexose-deoxyhexose. However, it should be noted that a few trisaccharides and monosaccharides were also identified in the study. The identification of some of these glycosides was further confirmed through direct correlation with the corresponding accumulated aroma compound. This study provides further evidence of the significant aromatic potential of winemaking grapes through their pool of aroma precursors, and it also suggests the possibility of exploring alternative methods for treating grapes to evaluate their aromatic potential.

Acknowledgements:

This work was funded by the Spanish Ministry of Science and Innovation (MICIN) (project AGL2017-87373-C3-1-R). E. S. A. has received a grant (PRE2018-084968) from the Spanish FPI programs associated to the same project. LAAE acknowledges the continuous support of Gobierno de Aragón (T29) and European Social Fund. 

References:

  1. Ferreira, V., & Lopez, R. (2019). The actual and potential aroma of winemaking grapes. Biomolecules, 9(12), 818. DOI 10.3390/biom9120818
  2. Loscos, N. et al. (2009). Comparison of the suitability of different hydrolytic strategies to predict aroma potential of different grape varieties. Journal of Agricultural and Food Chemistry, 57(6), 2468–2480. DOI 10.1021/jf803256e

DOI:

Publication date: October 5, 2023

Issue: ICGWS 2023

Type: Article

Authors

Elayma Sánchez-Acevedo, Marie Denat, Ignacio Ontañón, Ricardo Lopez, Vicente Ferreira

Laboratory for Flavor Analysis and Enology (LAAE), Department of Analytical Chemistry, Faculty of Sciences, Universidad de Zaragoza, Instituto Agroalimentario de Aragón-IA2 (Universidad de Zaragoza-CITA), Associate Unit to Instituto de las Ciencias de la Vid y del Vino (ICVV) (UR-CSIC-GR), E-50009 Zaragoza, Spain

Contact the author*

Keywords

grapes, glycosidic precursors, fast fermentation, acid hydrolysis, wine aging, wine aroma

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

The interplay between water deficit and nitrogen and potassium nutrition in Vitis vinifera L.

Climate change is expected to provoke an increase in the frequency and intensity of drought events and water scarcity that will have detrimental effects on photosynthesis and plant yield. To sustain an appropriate plant yield under sub-optimal conditions, a common practice is the application of high amounts of fertilizers with negative environmental consequences. The present study aims at evaluating the interplay between water and nutrient availability, namely nitrogen (N) and potassium (K), in two grapevine cultivars with a different sensitivity to water shortage stress. Two-year-old Vitis Vinifera cv. Cabernet Sauvignon and Grenache grapevine plants grafted on SO4 rootstock have been transferred in pots under semi-environmental conditions.

Late pruning, an alternative for rainfed vine varieties facing new climatic conditions

In Chile there is a dry farming area known as a traditional wine region, where varieties brought by the Spanish conquerors still persist. These varieties, in general, are cultivated under traditional systems, with low use of technical and economic resources, and low profitability for their grapes and wines. In this region, as in other wine grape growing areas, climatic conditions have changed significantly in recent decades. In particular, the occurrence of spring frosts, when bud break has already begun, have generated significant losses for these growers.

Entomopathogenic nematodes application for controlling Lobesia botrana in grapevine and their impact on grapevine quality 

Entomopathogenic nematodes (EPN) are well-known biological control agents combined with specific adjuvants that now allow their use against aerial pests. Lobesia botrana (Lepidoptera: Tortricidae) is one of the major harmful pests detected in worldwide vineyards. Previous studies demonstrated that the EPNs Steinernema feltiae and S. carpocapsae could control L. botrana. The hypothesis was that the best combination of EPN-adjuvant/timing (season/temperatures) will support the use of EPN in the vineyard against L. botrana with no impact on the grape performance.

Effect of pH and ethanol on Lactiplantibacillus plantarum in red must fermentation: potential use of wine lees

Wine is the result of the alcoholic fermentation (AF) of grape must. Besides AF, wine can also undergo the malolactic fermentation (MLF) driven out by lactic acid bacteria (LAB). Among LAB, Oenococcus oeni and Lactiplantibacillus plantarum are the dominant species in wine. Even if O. oeni is the most common LAB undergoing MLF in wine, due to its high tolerance to wine conditions, L. plantarum can be used to undergo MLF in must. The moderate tolerance of L. plantarum to low pH and ethanol, may compromise the fermentative process in harsh wines.

Effect of two water deficit regimes on the agronomic response of 12 grapevine varieties cultivated in a semi-arid climate

The Mediterranean basin is one of the most vulnerable regions to Climate Change effects. According to unanimous forecasts, the vineyards of Castilla-La Mancha will be among the most adversely affected by rising temperatures and water scarcity during the vine’s vegetative period. One potential strategy to mitigate the negative impacts of these changes involves the identification of grapevine varieties with superior water use efficiency, while ensuring satisfactory yields and grape quality.