terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Stomatal abundance in grapevine: developmental genes, genotypic variation, and physiology

Stomatal abundance in grapevine: developmental genes, genotypic variation, and physiology

Abstract

Grapevine cultivation is threatened by the global warming, which combines high temperatures and reduced rainfall, impacting in wine quality and even plant survival. Breeding for varieties resilient to these challenges must address plant traits such as tolerance to supraoptimal temperatures and optimized water use efficiency while minimizing productivity and quality losses. Stomatal abundance (SA) determines the maximum leaf potential for transpiration and thus water loss and cooling. Since SA results from a developmental process during leaf emergence and growth, knowledge on the genetic control of this process would provide specific targets for modification. Several genes controlling stomatal development have been characterized in Arabidopsis. Using translational genomics, we identified in the grapevine reference genome single orthologues of the master stomatal development regulators SPCH, MUTEand FAMA. We complemented Arabidopsis loss-of-function mutants with the grapevine candidate proteins, whose conditional overexpression also produced the expected epidermal phenotypes. The corresponding grapevine gene promoters are also under study. Additionally, we scored SA in 13 grapevine varieties over four consecutive growing seasons, and disclosed substantial SA differences with a strong genetic basis. These varieties were also examined for water use efficiency and physiological performance under drought and irrigation, finding significant varietal differences. Correlating developmental and physiological traits will contribute useful tools for grapevine management and breeding.   

Acknowledgements: This work was supported by the PID2019-105362RB-100, SBPLY/21/180501/000144, UCLM intramural grants and EU FEDER funds. PhD grants from JCCM supported AO and JIM.

DOI:

Publication date: October 6, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Mena-Morales A.1*, Martín-Forero A.F.2, Ortega A.2, Saiz-Pérez J.2, Martínez-Gascueña J.1, Chacón-Vozmediano J.L.1, Illescas-Miranda J.2, Fenoll C.2, Mena M. 2

1Regional Institute of Agri-Food and Forestry Research and Development of Castilla-La Mancha (IRIAF), IVICAM, Ctra. Toledo-Albacete s/n, 13700 Tomelloso (Ciudad Real), Spain
2 Faculty of Environmental and Biochemistry Sciences, University of Castilla-La Mancha, Avda. Carlos III, s/n, Technological Campus of the Arms Factory, 45071 Toledo, Spain

Contact the author*

Keywords

stomatal abundance, stomatal genes, genotypic variation, water use efficiency

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Can soil nitrate explain polyphenol and anthocyanin content in vineyard with similar available soil water regime? 

Nitrogen (N) is quite important nutrient in grapevine development and must quality, but under Mediterranean climatic conditions, available soil water (ASW) during grapevine development can also influence vigour and must quality. The aim was to determine the influence of soil nitrate (NO3-) availability on N foliar, yield, and must quality in vineyards with similar available water holding capacity (AWC). For this purpose, four cv. Tempranillo (Vitis vinifera L.) vineyards were selected. All of them are placed in Uruñuela municipality (La Rioja, Spain), separated less than 2.5 km and in a slope <1 %, in soils with similar soil chemistry properties and with similar rooting depth (ranging between 105 cm and 110 cm).

Towards a better understanding of cultivar susceptibility to esca disease: results from a pluriannual common garden monitoring

Grapevine (Vitis vinifera L.) exhibits a high level of genetic and phenotypic diversity among the approximately 6000 cultivars recorded. This perennial crop is highly vulnerable to numerous fungal diseases, including esca, which is a complex vascular pathology that poses a significant threat to the wine sector, as there is currently no cost-efficient curative method[1]. In this context, an effective approach to mitigate the impact of such diseases is by leveraging the crop’s genetic diversity. Indeed, susceptibility to esca disease appears to vary between cultivars, under artificial or natural infection. However, the mechanisms and varietal characteristics underlying cultivar susceptibility to esca are still unknown.

Investigation of cellulose nanofiber-based films used as a protective layer to reduce absorption of smoke phenols into wine grapes

Volatile phenols from wildfire smoke are absorbed by wine grapes, resulting in undesirable smoky and ashy sensory attributes in the affected wine.[1] Unfortunately the severity of wildfires is increasing, particularly when grapes are ripening on the vine. The unwanted flavors of the wine prompted a need for solutions to prevent the uptake of smoke compounds into wine grapes. Films using cellulose nanofibers as the coating forming matrix were developed as an innovative means to prevent smoke phenols from entering Pinot noir grapes. Different film formulations were tested by incorporating low methoxy pectin or chitosan.

Volatilome in grapevine leaves is defined by the variety and modulated by mycorrhizal symbiosis

Volatile organic compounds (VOCs) constitute a diverse group of secondary metabolites key for the communication of plants with other organisms and for their adaptation to environmental and biotic stresses. The emission of these compounds through leaves is also affected by the interaction of plants with symbiotic microorganisms, arbuscular mycorrhizal fungi (AMF) among them [1]. Our objective was to know the concentration and profile of VOCs emitted by the leaves of two grapevine varieties (Tempranillo, T, and Cabernet Sauvignon, CS, grafted onto R110 rootstocks), inoculated or not with a consortium of five AMF (Rhizophagus irregularis, Funneliformis mosseae, Septoglomus deserticola, Claroideoglomus claroideum and C. etunicatum).

Volatile composition of Cabernet Sauvignon wines from Argentina, Portugal and Spain

Cabernet Sauvignon is one of the most cultivated grape varieties worldwide being grown in different environmental conditions due to its excellent adaptability. Volatile compounds deeply contribute to the sensory properties of wines therefore to wine quality. The aim of this work was to compare the aroma profile of Cabernet Sauvignon wines from different geographical areas and climatic conditions, namely from Argentina, Portugal and Spain, from the vintage 2022. In addition, the volatile composition of the Cabernet Sauvignon Portuguese wines from three vintages was evaluated.