terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Stomatal abundance in grapevine: developmental genes, genotypic variation, and physiology

Stomatal abundance in grapevine: developmental genes, genotypic variation, and physiology

Abstract

Grapevine cultivation is threatened by the global warming, which combines high temperatures and reduced rainfall, impacting in wine quality and even plant survival. Breeding for varieties resilient to these challenges must address plant traits such as tolerance to supraoptimal temperatures and optimized water use efficiency while minimizing productivity and quality losses. Stomatal abundance (SA) determines the maximum leaf potential for transpiration and thus water loss and cooling. Since SA results from a developmental process during leaf emergence and growth, knowledge on the genetic control of this process would provide specific targets for modification. Several genes controlling stomatal development have been characterized in Arabidopsis. Using translational genomics, we identified in the grapevine reference genome single orthologues of the master stomatal development regulators SPCH, MUTEand FAMA. We complemented Arabidopsis loss-of-function mutants with the grapevine candidate proteins, whose conditional overexpression also produced the expected epidermal phenotypes. The corresponding grapevine gene promoters are also under study. Additionally, we scored SA in 13 grapevine varieties over four consecutive growing seasons, and disclosed substantial SA differences with a strong genetic basis. These varieties were also examined for water use efficiency and physiological performance under drought and irrigation, finding significant varietal differences. Correlating developmental and physiological traits will contribute useful tools for grapevine management and breeding.   

Acknowledgements: This work was supported by the PID2019-105362RB-100, SBPLY/21/180501/000144, UCLM intramural grants and EU FEDER funds. PhD grants from JCCM supported AO and JIM.

DOI:

Publication date: October 6, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Mena-Morales A.1*, Martín-Forero A.F.2, Ortega A.2, Saiz-Pérez J.2, Martínez-Gascueña J.1, Chacón-Vozmediano J.L.1, Illescas-Miranda J.2, Fenoll C.2, Mena M. 2

1Regional Institute of Agri-Food and Forestry Research and Development of Castilla-La Mancha (IRIAF), IVICAM, Ctra. Toledo-Albacete s/n, 13700 Tomelloso (Ciudad Real), Spain
2 Faculty of Environmental and Biochemistry Sciences, University of Castilla-La Mancha, Avda. Carlos III, s/n, Technological Campus of the Arms Factory, 45071 Toledo, Spain

Contact the author*

Keywords

stomatal abundance, stomatal genes, genotypic variation, water use efficiency

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Combined abiotic-biotic plant stresses on the roots of grapevine

In the 19th century, devastating outbreaks of phylloxera (Daktulosphaira vitifoliae Fitch), almost brought European viticulture to its knees. Phylloxera does not only take energy in form of sugars from the vine, but also affects the up- and down- regulations of genes, acts as a carbon sink and reprograms the physiology of the grapevines, including nutrient uptake and the defense system [1]. A key trait of rootstocks is the ability to perform well under high lime conditions as about 30 % of the land surface has calcareous soil. Iron deficiency not only causes the well-known problems of lime-induced chlorosis and stunted growth, but also affects the entire plant metabolism.

Comparison of ancestral and traditional methods in the elaboration of sparkling wines; preliminary results

Top quality sparkling wines (SW) are mostly produced using the traditional method that implies a second fermentation into the bottle[1]. That is the case of sparkling wines of reputed AOC such as Champagne, Cava or Franciacorta. However, it seems that the first SW was elaborated using the ancestral method in which only one fermentation takes place[2]. That is the case of the classical SW from the AOC Blanquette de Limoux[3]. In both cases, SW age in the bottle during some time in contact with lees favoring yeast’s autolysis[4]. There is a lot of information about traditional method but only few exists about ancestral method. The aim of this work was to compare SW made by the ancestral method with SW made by the traditional method.

High-throughput sequencing analysis based on nematode indices revealed healthier soils of organic vineyards 

Proper soil health assessments are crucial for sustainable cropland. Among the widely employed approaches, evaluating nematode community structure is particularly suitable. Traditionally, the taxonomic characterization of soil nematodes has relied on time-consuming morphology-based methods requiring experienced experts. However, molecular tools like high-throughput sequencing have emerged as efficient alternatives. In this study, we performed a metataxonomic analysis of soil samples collected from 57 vineyards in the DOCa Rioja region of Northern Spain, focusing on the impact of organic viticulture and cover cropping compared to integrated pest management (IPM) and tilling practices.

Chemical and microbiological evaluation of Ribeiro wines (NW Spain)

Wine produced under Designation of Origin (DOP) Ribeiro, the oldest DOP in Galicia (NW Spain), are elaborated using local grape cultivars, grown at the valleys of Miño, Avia and Arnoia rivers. The landscape formed by slopes and terraces and the peculiar climate of continental character, softened by the proximity of Atlantic Ocean, make it an area of excellent aptitude for vine cultivation. In addition, small-scale farming and the use of traditional techniques for vineyard management provide a great diversity to Ribeiro wines. This study presents the evaluation of red and white wines (bottled or bulk wines) from DOP Ribeiro, produced between years 2018-2022.

Wine without added SO2: Oxygen impact and color evolution during red wine aging

SO2 play a major role in wine stability and evolution during its aging and storage. Winemaking without SO2 is a big challenge for the winemakers since the lack of SO2 affects directly the wine chemical evolution such as the aromas compounds as well as the phenolic compounds. During the red wine aging, phenolic compounds such as anthocyanin, responsible of the red wine colour, and tannins, responsible of the mouthfeel organoleptic properties of wine, evolved quickly from the winemaking process to aging [1]. A lot of new interaction and molecules occurred lead by oxygen[2] thus the lack of SO2 will induce wine properties changes. Nowadays, the phenolic composition of the wine without added SO2 have not been clearly reported.