terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Stomatal abundance in grapevine: developmental genes, genotypic variation, and physiology

Stomatal abundance in grapevine: developmental genes, genotypic variation, and physiology

Abstract

Grapevine cultivation is threatened by the global warming, which combines high temperatures and reduced rainfall, impacting in wine quality and even plant survival. Breeding for varieties resilient to these challenges must address plant traits such as tolerance to supraoptimal temperatures and optimized water use efficiency while minimizing productivity and quality losses. Stomatal abundance (SA) determines the maximum leaf potential for transpiration and thus water loss and cooling. Since SA results from a developmental process during leaf emergence and growth, knowledge on the genetic control of this process would provide specific targets for modification. Several genes controlling stomatal development have been characterized in Arabidopsis. Using translational genomics, we identified in the grapevine reference genome single orthologues of the master stomatal development regulators SPCH, MUTEand FAMA. We complemented Arabidopsis loss-of-function mutants with the grapevine candidate proteins, whose conditional overexpression also produced the expected epidermal phenotypes. The corresponding grapevine gene promoters are also under study. Additionally, we scored SA in 13 grapevine varieties over four consecutive growing seasons, and disclosed substantial SA differences with a strong genetic basis. These varieties were also examined for water use efficiency and physiological performance under drought and irrigation, finding significant varietal differences. Correlating developmental and physiological traits will contribute useful tools for grapevine management and breeding.   

Acknowledgements: This work was supported by the PID2019-105362RB-100, SBPLY/21/180501/000144, UCLM intramural grants and EU FEDER funds. PhD grants from JCCM supported AO and JIM.

DOI:

Publication date: October 6, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Mena-Morales A.1*, Martín-Forero A.F.2, Ortega A.2, Saiz-Pérez J.2, Martínez-Gascueña J.1, Chacón-Vozmediano J.L.1, Illescas-Miranda J.2, Fenoll C.2, Mena M. 2

1Regional Institute of Agri-Food and Forestry Research and Development of Castilla-La Mancha (IRIAF), IVICAM, Ctra. Toledo-Albacete s/n, 13700 Tomelloso (Ciudad Real), Spain
2 Faculty of Environmental and Biochemistry Sciences, University of Castilla-La Mancha, Avda. Carlos III, s/n, Technological Campus of the Arms Factory, 45071 Toledo, Spain

Contact the author*

Keywords

stomatal abundance, stomatal genes, genotypic variation, water use efficiency

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Exploring intra-vineyard variability with sensor- and molecular-based approaches 

The application of remote and proximal sensing is a fast and efficient method to monitor grapevine vegetative and physiological parameters and is considered valuable to derive information on associated yield and quality traits in the vineyard. Further details can be obtained by the application of molecular analysis at the gene expression level aiming at elucidating how pathways controlling the formation of different grape quality traits are influenced by spatial variability. This work aims at evaluating intra-vineyard variability in grape composition at harvest and at comparing this with remotely sensed canopy vegetation data and molecular-based approaches.

Options to replace or reduce the sulphite content in Tannat red wines produced with minimal intervention

Several Uruguayan wineries have begun to produce wines with minimal intervention, to increase the sustainability of their vineyards and wines. These wines are characterized by the minimum intervention in the management of the vineyard, its harvest, vinification, conservation and aging1,2. Sulfur dioxide (SO2) is not used or is used in reduced doses, although chitosan can be substituted or supplemented1. The objective of this research is to evaluate SO2 reduction or replacement options adapted to the production of Tannat red wines with minimal intervention. Vinification of the Tannat grapes with autochthonous yeasts (LN) was carried out during the 2023 vintage.

Differences in metabolism among species and hybrids of the genus Saccharomyces during wine fermentation unveiled by multi-omic analysis 

Yeast species S. cerevisiae, S. uvarum, S. kudriavzevii and their hybrids present clear metabolic differences, even when we compared S. cerevisiae wine versus wild strain. These species and hybrids produced significantly higher amounts of glycerol, organic acids, 2,3-butanediol, and 2-phenyl ethanol and a reduction of the ethanol yield, properties very interesting in the sector to deal with climate change effects. To understand the existing differences, we have used several omics techniques to analyze the dynamics of the (intra- and extracellular) metabolomes and/or transcriptomes of representative strains of S. cerevisiae, S. uvarum, S. kudriavzevii, and hybrids.

Combined use of leaf removal and natural shading to delay grape ripening in Manto negro (Vitis vinifera L.) under deficit irrigation 

The increasingly frequent heat waves during grape ripening pose challenges for premium wine grape production. This makes the development of irrigation and canopy management techniques of great importance to maximize yield and grape quality. A field experiment was carried out during 2021 and 2022 using Manto negro wine grapes to study the effect of two irrigation strategies and different light exposure levels on grape quality.

Molecularly imprinted polymers: an innovative strategy for harvesting polyphenoles from grape seed extracts

Multiple sclerosis (MS) is a multifactorial autoimmune disease associating demyelination and axonal degeneration developing in young adults and affecting 2–3 million people worldwide. Plant polyphenols endowed with many therapeutic benefits associated with anti-inflammatory and antioxidant properties represent highly interesting new potential therapeutic strategies. We recently showed the safety and high efficiency of grape seed extract (GSE), a complex mixture of polyphenolics compounds comprising notably flavonoids and proanthocyanidins, in an experimental autoimmune encephalomyelitis (EAE) mouse model of MS.