terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Accumulation of deleterious mutations in grapevine and its relationship with traits of interest for wine production and resilience

Accumulation of deleterious mutations in grapevine and its relationship with traits of interest for wine production and resilience

Abstract

Deleterious mutations that severely reduce population fitness are rapidly removed from the gene pool by purifying selection. However, evolutionary drivers such as genetic drift brought about by demographic bottlenecks may comprise its efficacy by allowing deleterious mutations to accumulate, thereby limiting the adaptive potential of populations. Moreover, positive selection can hitchhike mildly deleterious mutations due to linkage caused by lack of recombination. Similarly, in the context of species domestication, artificial selection mimics these evolutionary processes, which can have undesirable consequences for production and resilience. In this study, we evaluated the extent of the accumulation of deleterious mutations and the magnitude of their effects (also known as genetic load) at the whole-genome scale for ca. 500 grapevines including both wild Vitis species originated in three geographical regions (North America, Asia and Europe), and commercial varieties of V. vinifera ssp. sativa (using Illumina paired-end sequencing 20x from the 4k project[1]). We comparatively estimated the overall efficacy of selection as the ratio of non-synonymous to synonymous mutations in protein coding regions. We also performed various functional prediction analyses on the genomic sequence data to identify deleterious alleles and their effect on gene expression to quantify genetic load. In addition, 40 of the sequenced grapevine varieties were evaluated in an experimental common garden in Bordeaux (VitAdapt[2]). We obtained phenotypic traits related to wine productivity and resilience (e.g., phenology, biomass growth, water use efficiency and grape production) which allowed to analyse the correlation between the estimated genetic load and phenotypes. The results obtained from this work will contribute to the understanding of how we can best account for deleterious alleles and genetic load in new-generation grapevine breeding.

Acknowledgements: This study received financial support from the French government in the framework of the IdEX Bordeaux University “Investments for the Future” program / GPR Bordeaux Plant Sciences, and from the University of Bordeaux (PurVitis project).

1)  Dong, Y.,et al. (2023). Dual domestications and origin of traits in grapevine evolution. Science, 379(6635), 892901.

2)  Destrac Irvine A. and van Leeuwen C. (2016) The VitAdapt project: extensive phenotyping of a wide range of varieties in order to optimize the use of genetic diversity within the Vitis vinifera species as a tool for adaptation to a changing environment. Climwine, sustainable grape and wine production in the context of climate change, 11-13 April 2016, Bordeaux. Full text proceedings paper, 165-171.

DOI:

Publication date: October 6, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Enrique Sáez-Laguna 1*, Gaëtan Craye 1Agnes Destrac Irvine 2, Cornelis van Leeuwen 2, Nabil Girollet 2, Pierre-François Bert 2, Nathalie Ollat 2, Santiago González-Martínez 1, Marina de Miguel 2

1 BIOGECO, Univ. Bordeaux, INRAE, 33610 Cestas, France
2 EGFV, Univ. Bordeaux, Bordeaux Sciences Agro, INRAE, ISVV, 33882 Villenave d’Ornon, France

Contact the author*

Keywords

deleterious mutations, genetic load, fitness, grape, variety, production

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Phenolic extraction and dissolved oxygen concentration during red wines fermentations with Airmixig M.I.™

During red wine fermentation, the extraction of phenolics compounds and sufficient oxygen provision are critical for wine quality [1,2]. In this trial, we aimed at evaluating the kinetics of phenolic extraction and dissolved oxygen during red wine fermentations using the airmixing system. Twenty lots of red grape musts were fermented in 300.000 L tanks, equipped with airmixing, using two injection regimes (i.e., high and low intensity, and high and low daily frequency). An oxygen analyzer was introduced into the tanks in order to record the concentration of dissolved oxygen over time.

Qualitative and productive characterization of a minority variety: ‘Branco lexítimo’ in DO Ribeira Sacra (Spain)

The actual climate changes, together with the strong regulation of the European Union and Spanish government, in search of sustainable viticulture, have forced the recovery of minority varieties, expanding the range of grape varieties, as well as the possible development of wines with unique profiles. In the Ribeira Sacra DO (Spain), a comparative study of the agronomic and qualitative behavior of the ‘Branco lexítimo’ variety has been carried out, compared to the majority white variety in the DO: ‘Godello’, located in the same study plot, with identic soil and climatic conditions. The study contemplated the analysis of phenology and leaf water potential, as well as the productive results and the analysis of the must quality, during four seasons: 2018 – 2021.

Combined use of leaf removal and natural shading to delay grape ripening in Manto negro (Vitis vinifera L.) under deficit irrigation 

The increasingly frequent heat waves during grape ripening pose challenges for premium wine grape production. This makes the development of irrigation and canopy management techniques of great importance to maximize yield and grape quality. A field experiment was carried out during 2021 and 2022 using Manto negro wine grapes to study the effect of two irrigation strategies and different light exposure levels on grape quality.

Effect of biological control agents on grapevine rhizosphere microbiome and grapevine defenses

Plant diseases are a major obstacle to crop production. The main approaches to battle plant diseases, consist of synthetic chemicals to attack infecting pathogens. However, concerns are increasing about the effects of chemicals in the environment, leading to an increase in the use of biocontrol agents (BCAs), due to their assets, such as, antagonism, and competition. In this study, we tested the hypothesis that the introduction of Bacillus subtilis PTA-271 (Bs PTA-271) and Trichoderma atroviride SC1 (Ta SC1) produce distinctive modifications in the composition and network structure of the grapevine rhizosphere microbial community, as well as grapevine induced defenses.

Conventional and alternative pest management strategies: a comparative proteomic study on musts

In a context of sustainable agriculture, “agroecological immunity” is an emerging concept to reduce the use of chemical pesticides to protect crops against pathogens. This alternative strategy aims to combine different levers including the use of “bio”solutions. These include biocontrol products, some of which being plant defense elicitors, as well as products authorized in organic farming such as copper or sulfur. In vineyards, depending on climate conditions, powdery and downy mildews can be devastating diseases.