terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Accumulation of deleterious mutations in grapevine and its relationship with traits of interest for wine production and resilience

Accumulation of deleterious mutations in grapevine and its relationship with traits of interest for wine production and resilience

Abstract

Deleterious mutations that severely reduce population fitness are rapidly removed from the gene pool by purifying selection. However, evolutionary drivers such as genetic drift brought about by demographic bottlenecks may comprise its efficacy by allowing deleterious mutations to accumulate, thereby limiting the adaptive potential of populations. Moreover, positive selection can hitchhike mildly deleterious mutations due to linkage caused by lack of recombination. Similarly, in the context of species domestication, artificial selection mimics these evolutionary processes, which can have undesirable consequences for production and resilience. In this study, we evaluated the extent of the accumulation of deleterious mutations and the magnitude of their effects (also known as genetic load) at the whole-genome scale for ca. 500 grapevines including both wild Vitis species originated in three geographical regions (North America, Asia and Europe), and commercial varieties of V. vinifera ssp. sativa (using Illumina paired-end sequencing 20x from the 4k project[1]). We comparatively estimated the overall efficacy of selection as the ratio of non-synonymous to synonymous mutations in protein coding regions. We also performed various functional prediction analyses on the genomic sequence data to identify deleterious alleles and their effect on gene expression to quantify genetic load. In addition, 40 of the sequenced grapevine varieties were evaluated in an experimental common garden in Bordeaux (VitAdapt[2]). We obtained phenotypic traits related to wine productivity and resilience (e.g., phenology, biomass growth, water use efficiency and grape production) which allowed to analyse the correlation between the estimated genetic load and phenotypes. The results obtained from this work will contribute to the understanding of how we can best account for deleterious alleles and genetic load in new-generation grapevine breeding.

Acknowledgements: This study received financial support from the French government in the framework of the IdEX Bordeaux University “Investments for the Future” program / GPR Bordeaux Plant Sciences, and from the University of Bordeaux (PurVitis project).

1)  Dong, Y.,et al. (2023). Dual domestications and origin of traits in grapevine evolution. Science, 379(6635), 892901.

2)  Destrac Irvine A. and van Leeuwen C. (2016) The VitAdapt project: extensive phenotyping of a wide range of varieties in order to optimize the use of genetic diversity within the Vitis vinifera species as a tool for adaptation to a changing environment. Climwine, sustainable grape and wine production in the context of climate change, 11-13 April 2016, Bordeaux. Full text proceedings paper, 165-171.

DOI:

Publication date: October 6, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Enrique Sáez-Laguna 1*, Gaëtan Craye 1Agnes Destrac Irvine 2, Cornelis van Leeuwen 2, Nabil Girollet 2, Pierre-François Bert 2, Nathalie Ollat 2, Santiago González-Martínez 1, Marina de Miguel 2

1 BIOGECO, Univ. Bordeaux, INRAE, 33610 Cestas, France
2 EGFV, Univ. Bordeaux, Bordeaux Sciences Agro, INRAE, ISVV, 33882 Villenave d’Ornon, France

Contact the author*

Keywords

deleterious mutations, genetic load, fitness, grape, variety, production

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Implications of the nature of organic mulches used in vineyards on grapevine water status, yield, berry quality and biological soil health  

Climate emergency is going to affect the agricultural suistainability, wine grapes being probably one of the crops more sensitive to environmental constraints. In this context, mitigation strategies such as the revalorization of agricultural wastes are paramount to cope with the current challenges. The use of organic mulches has been reported to reduce soil water evaporation and improve vine water status, reduce soil erosion, and increase soil organic matter with little impact on berry quality. However, less is known about their effects on the microbiote of vineyards.

Foliar application of urea improved the nitrogen composition of Chenin grapes

The nitrogen composition of the grapes directly affects the developments of alcoholic fermentation and influences the final aromatic composition of the wines. The aim of this study was to determine the effect and efficiency of foliar applications of urea on the nitrogen composition of grapes. This study was carried out during 2023 vintage and in the Chenin vineyard located in Estacion Experimental Mendoza (Argentina). Three urea concentrations 3, 6 and 9 Kg N/ha (C1, C2, and C3, respectively) and control (T) were applied in this vineyard at veraison.

Adsorption of tetraconazole by organic residues and vineyard organically-amended soils 

Spain is the country with the largest wine-producing area in the EU and its productivity is largely controlled applying fungicides. However, residues of these compounds can move and contaminate surface and groundwater. The objective of this work was to evaluate the capacity of bioadsorbents from different origin to adsorb and immobilize tetraconazole by themselves or when applied as organic soil amendment, and to prevent soil and water contamination by this fungicide. The adsorption of tetraconazole by 3 organic residues: spent mushroom substrate (SMS), green compost (GC) and vine pruning sawdust (VP), as well as by vineyard soils unamended and amended individually with these residues at 1.5% (w/w) was evaluated using the batch equilibrium technique.

Pre-breeding for developing heat stress resilient grape varieties to ensure yield 

Climate change has numerous detrimental consequences and creates new challenges for viticulture around the world. Transitory or constant high temperatures frequently associated with an excess of sunlight (UV) can cause a variety of physiological disorders, such as sunburn. Diverse environmental factors and the plant’s response mechanisms to stress determine the symptoms. Grapevine berry sunburn leads to a drastic reduction in yield, and may eventually decline berry quality. Consequently, this poses a significant risk to the winegrowers.

Retrospective analysis of our knowledge regarding the genetics of relevant traits for rootstock breeding 

Rootstocks were the first sustainable and environmentally friendly strategy to cope with a major threat for Vitis vinifera cultivation. In addition to providing Phylloxera resistance, they play an important role in protecting against other soil-borne pests, such as nematodes, and in adapting V. vinifera to limiting abiotic conditions. Today viticulture has to adapt to ongoing climate change whilst simultaneously reducing its environmental impact. In this context, rootstocks are a central element in the development of agro-ecological practices that increase adaptive potential with low external inputs. Despite the apparent diversity of the Vitis genus, only few rootstock varieties are used worldwide and most of them have a very narrow genetic background. This means that there is considerable scope to breed new, improved rootstocks to adapt viticulture for the future.