terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Do wine sulphites affect gut microbiota? An in vitro study of their digestion in the gastrointestinal tract

Do wine sulphites affect gut microbiota? An in vitro study of their digestion in the gastrointestinal tract

Abstract

“Sulphites” and mainly sulphur dioxide (SO2) is by far the most widely used additive (E-220/INS 220) in winemaking and likely the most difficult to replace. The well-known antioxidant, antioxidasic and antimicrobial properties of SO2 make this molecule a practically essential tool, not only in winemaking, but also in the production of other food products. The current trend in winemaking is the reduction of this unfriendly additive due to its negative effects on health and environmental. In particular, it could cause headaches and intolerance/allergic reactions in sensitive individuals. Wine is considered one of the major contributors of exposure of SO2 in the adult population, when this beverage is included in the diet. The European Union establishes that the limits for total SO2 content may not exceed 200 mg/L for red wines with a sugar content higher than 5 g/L, whereas the threshold for an adverse reaction varies between 5 and 200 mg/L SO2. The gut microbiome is now considered a therapeutic target for many pathologies and for general health status. Recent research has highlighted the potential of wine to modulate the gut microbiome, mainly attributed to its phenolic content and diversity. To our knowledge, very few studies have addressed the effects of sulphites on the gut microbiota, which could be mediated by the dietary matrix. Therefore, the novel question that arises is whether the presence of sulphites in wine may also affect our gut microbiome. To disclose this matter, we have designed an in vitro study based on the simulated gastrointestinal digestion in the simgi® simulator of the following comparative wines: a) synthetic wine, b) synthetic wine fortified with SO2 (200 mg/L), c) young red wine (2,8 mg/L of free SO2), and d) young red wine fortified with SO2 (200 mg/L). The following analyses were performed in the wines after intestinal and colonic (0, 6, 24, and 48h) digestions: free and bound SO2 by the PAUL-Rankine method (OIV-MA-AS323-04A), microbial plate counting, qPCR and 16S rDNA sequencing, microbial ammonium production, short chain fatty acids (SCFA) by SMPE-GCMS, and phenolic metabolites by UPLC-ESI-MSMS. The results indicate that, at least to some extent, the addition of sulphites to wine may have an impact on the gut microbiome, although this may be dependent on the composition of the wine, especially with regard to its phenolic content.

Acknowledgements: MICIN (PID2019-108851RB-C21 Project). The authors would also like to thank R. de Diego for sound technical assistance.

DOI:

Publication date: October 16, 2023

Issue: ICGWS 2023

Type: Poster

Authors

E. Relaño de la Guia1, C. Cueva1, N. Molinero1, M.J.Motilva2,  B. Bartolomé1, M.V. Moreno-Arribas

1Institute of Food Science Research (CIAL), CSIC-UAM, 28049 Madrid, Spain
2Institute of Grapevine and Wine Sciences (ICVV), CSIC-University of La Rioja-Government of La Rioja, 26007 Logroño (La Rioja), Spain

Contact the author*

Keywords

wine, SO2, gut microbiome, 16S rDNA sequencing, SCFA, phenolic metabolites

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Response of red grape varieties irrigated during the summer to water availability at the end of winter in four Spanish wine-growing regions: berry phenolic composition

Water availability is the most limiting factor for vineyard productivity under Mediterranean conditions. Due to the effects caused by the current climate change, wine-growing regions may face serious soil moisture conservation problems, due to the lower water retention capacity of the soil and higher soil irradiation. The aim of this work was to evaluate the effects of soil recharge irrigation in pre-sprouting and summer irrigation every week (30 % ETo) from the pea size state until the end of ripening (RP) compared to exclusively summer irrigation every week (R) in the same way that RP, on berry phenolic composition at harvest.

Qualitative and productive characterization of a minority variety: ‘Branco lexítimo’ in DO Ribeira Sacra (Spain)

The actual climate changes, together with the strong regulation of the European Union and Spanish government, in search of sustainable viticulture, have forced the recovery of minority varieties, expanding the range of grape varieties, as well as the possible development of wines with unique profiles. In the Ribeira Sacra DO (Spain), a comparative study of the agronomic and qualitative behavior of the ‘Branco lexítimo’ variety has been carried out, compared to the majority white variety in the DO: ‘Godello’, located in the same study plot, with identic soil and climatic conditions. The study contemplated the analysis of phenology and leaf water potential, as well as the productive results and the analysis of the must quality, during four seasons: 2018 – 2021.

The colour pattern of flower arrangements influence wine tasters’ sensory description

The arrangements of flowers and wine counterparts are inextricably linked. Whether a fundamental aspect of tablescaping or acolytes to broader entertainment rituals, they have an entangled history since ancient times. The aim of this contribution is to verify the influence of visually delicate and robust flower arrangements on individual description of wines. Changes in the sensory description of wines were investigated during subjects’ (thirty-two participants) exposure to three different conditions: the presence of delicate, robust, or totally absent flower arrangements. In each condition, the same two wines were blind tasted: a wine previously defined as delicate – a Pinot Noir from Australia, and a wine known for its robust character – a Tannat from Uruguay.

Evaluation of interception traps for capture of Xylotrechus arvicola (Coleoptera: Cerambycidae) in vineyards varieties from Protected Denomination of Origin León

Xylotrechus arvicola (Coleoptera: Cerambycidae) is a pest in vineyards (Vitis vinifera) in the main Spain wine-producing regions with Protected Denomination of Origin (PDO). The action of the larvae, associated to the spreading of wood fungi, causes damage especially in important varieties of V. vinifera. X. arvicola females lay eggs concentrated in cracks or under the rhytidome in the wood vines, which allows the emerging larvae to get into the wood and make galleries inside the plant being then necessary to prune intensively or to pull up the bored plants (1). The objective of the study was to evaluate captures of X. arvicola insects in five varieties of V. vinifera in PDO León.

Teinturier grapes: Valorization as a source of high-value compounds for the Chilean food industry

The agri-food industry is constantly searching for ingredients of high functional value, healthy and of natural origin. One species of particular interest is Vitis vinifera, due to its recognized antioxidant potential. Among the grape varieties, one group possesses these antioxidant compounds not only in the skin, but also in its pulp: Teinturier. The red grape has traditionally been used for color correction purposes in winemaking, however, its high antioxidant content transforms it into a raw material of high potential for new formulations of ingredients and foods for the health and wellness market.