Which potential for Near Infrared Spectroscopy to characterize rootstock effects on grapevines?

Cis-acting regulatory elements are DNA sequences that can be bound by transcription factors to regulate the expression of genes in a condition-dependent and tissue-specific way. It is nowadays possible to search for DNA motives and sequences that a given transcription factor is binding or at least can, but it is still hard to have a glance at all the transcription factors that are contemporaneously located at the same locus. Inspired by an existing technique that uses the CRISPR-Cas system in mammal cells, we are trying to develop a protocol to study such regulation in Vitis vinifera. Using the highly sequence-specific binding capacity of a catalytically inactive Cas9 protein (dCas9), our idea is to set up a system to target a desired sequence and precipitate all the crosslinked proteins and distantly interacting chromatin at this locus and analyze them.

Climatic variability studies at fine scale have been developed in recent years with the reduction of material cost and the development of competitive miniaturized sensors. This work is forming part the LIFE-ADVICLIM project, of which one of the objectives is to model spatial temperature variability at vineyard scale. In the Bordeaux pilot site, a large network of data loggers has been set up to record temperature close to the vine canopy. The reduced distance between plant foliage and measurement equipment raises specific issues and leads to an increased rate of outliers compared to data retrieved from classical weather stations. Some of these were detected during data analysis, but others could not be easily identified. The present study aims to address the issue of data quality control and provide recommendations for data processing in climatic studies at fine scale.

Grapevine (vitis spp.) Is one of the major and most economically important fruit crops worldwide. Unlike other cropping systems, viticulture has ancient historical connections with the development of human culture and with the socio-cultural background of grape-growing areas. The vitis genus is characterised by high levels of genetic diversity, as result of natural genetic mutations, which are common in grapevines and further assisted by ongoing vegetative propagation.

In recent years, resistant varieties have returned to the attention of the wine sector as a response to climate change and the reduction of pesticides in grapevine management, which is the main culprit of pesticide use in European agriculture. In this context, the production of sparkling wines could be strongly influenced due to its requirements for a particular balance between sugars and acidity, and the necessity of sound grapes to ensure wine quality. However, these parameters are not the only ones that define the suitability of a grape variety to produce sparkling wine.

Le Dipartimento di Progettazione dell ‘Architettura del Politecnico di Milano et l’Istituto di Viticoltura della Facoltà d’Agraria di Piacenza dell’Università Cattolica del Sacra Cuore, ont elaboré une proposition pour réaliser, dans l’aire de colline de la province de Piacenza, un Parco Produttivo Agrovitivinicolo.