terclim by ICS banner
IVES 9 IVES Conference Series 9 VviSOC1a and VviAG1 act antagonistically in the regulation of flower formation

VviSOC1a and VviAG1 act antagonistically in the regulation of flower formation

Abstract

The SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1) is a key floral activator that coordinates external and internal stimuli to ensure timely flowering. During early stages of flower formation, SOC1 represses floral organ identity genes such as AGAMOUS (AG) to prevent premature organ differentiation. In addition to floral organ specification, AG has been shown to regulate fleshy fruit expansion and ripening and, as such, is an important contributor to fruit quality traits. Currently, little is known about the function and gene regulatory network of the grapevine homologs VviSOC1a and VviAG1. As such, the aim of this study was to functionally characterise both genes by overexpressing them in tomato and performing phenotypic and gene expression studies. A dual luciferase (DL) assay involving putative target gene promoters was also conducted. Overexpression of VviSOC1a led to the development of leaf-like sepals, petals with increased chlorophyll content and plant sterility phenotypes. VviAG1-OE lines displayed hastened floral initiation, stamenoid petals, dwarfed fruit, as well as forming fleshy fruit sepals which gave the appearance of ripened pericarp tissue. The observed floral phenotypes were, in part, supported by the modulation of genes required for floral organ specification in tomato. VviSOC1a and VviAG1 displayed opposite expression trends, while also repressing each other’s expression in the DL assay. Collectively, the findings of this study supported a role for VviSOC1a in regulating floral organ specification, through the repression of the stamen and carpel identity gene VviAG1. An additional function for VviAG1 in berry development and ripening is also suggested.

Download the abstract

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Jenna Jolliffe1,2, Claudio Moser2, Stefania Pilati2 and Justin Lashbrooke3*

1South African Grape and Wine Research Institute, Stellenbosch University, Stellenbosch, 7600, South Africa
2Research and Innovation Centre, Edmund Mach Foundation, San Michele all’Adige, 38098, Italy
3Department of Genetics, Stellenbosch University, Stellenbosch, 7600, South Africa

Contact the author*

Keywords

SOC1, AG1, Flower, transcription factor, development

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Green pruning of shoots to force new sprouting of buds, in fruit set and in pea size: vegetative, productive and maturation effects, in cv. Verdejo

The context of climate crisis leads to the acceleration of technological ripening of grapes, with unsuitable loss of acidity, so various vineyard management alternatives are being considered to delay the grape ripening. The delay of the vegetative cycle towards a period of milder temperatures affects ripening, but vine behavior can vary according to the area, conduction, watering, variety, etc. A work is proposed to know the response to the green pruning of shoots, executed in fruit set and in pea size, in cv. Verdejo.

Read More

Control of bacterial growth in carbonic maceration winemaking through yeast inoculation

Controlling the development of the bacterial population during the winemaking process is essential for obtaining correct wines[1]. Carbonic Maceration (CM) wines are recognised as high-quality young wines. However, due to its particularities, CM winemaking implies a higher risk of bacterial growth: lower SO2 levels, enrichment of the must in nutrients, oxygen trapped between the clusters… Therefore, wines produced by CM have slightly higher volatile acidity values than those produced by the destemming/crushing method[2].

Read More

Evolution of cabernet sauvignon wines macerated with their own toasted vine-shoots

Toasted pruning vine-shoots represent a promising new enological tool for developing wines with chemical and organoleptic high quality, allowing that the resources of the vineyard to be returned to the wine through a “circular process”.

Read More

Removal of Fumonisin B1 and B2 from red wine using polymeric substances

The Ability of PVPP (Polyvinylpolypyrrolidone), PVP-DEGMA-TAIC (copolimerization of N-vinyl-2-pyrrolidinone with ethylene glycol dimethacrylate and triallyl isocyanurate) and PAEGDMA
(poly(acrylamide-co-ethylene glycol dimethacrylate)) polymers was tested as removal agents for Fumonisin B1 (FB1) and Fumonisin B2 (FB2) from model solutions and red wine. The polymers removal capacity was checked at three different resident times (2, 8 and 24 hours of contact time between the polymer and the sample), showing no differences in the percentage of FB1 and FB2 removal. Then, different polymer concentrations (1, 5 and 10 mg mL-1) were tested in model solution with and without phenolics (i.e. gallic acid and 4-methylcatechol).

Read More

Optimization of in vitro establishment of grapevine varieties for fast micropropagation 

Micropropagation is an important alternative to conventional methods of plant propagation. The objective of this study was to optimize a protocol for in vitro micropropagation of selected grapevine hybrids (H19 and H20) that are included in our breeding program. For the sprouting initiation experiment, nodal cuttings with only one axillary bud from two hybrids were separated, disinfected, and cultivated in 50% Murashige Skoog nutrient medium (½ MS) and Woody Plant Medium (WPM), adding 4.4 µM benzyladenine (BA) in both mediums.

Read More