terclim by ICS banner
IVES 9 IVES Conference Series 9 VviSOC1a and VviAG1 act antagonistically in the regulation of flower formation

VviSOC1a and VviAG1 act antagonistically in the regulation of flower formation

Abstract

The SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1) is a key floral activator that coordinates external and internal stimuli to ensure timely flowering. During early stages of flower formation, SOC1 represses floral organ identity genes such as AGAMOUS (AG) to prevent premature organ differentiation. In addition to floral organ specification, AG has been shown to regulate fleshy fruit expansion and ripening and, as such, is an important contributor to fruit quality traits. Currently, little is known about the function and gene regulatory network of the grapevine homologs VviSOC1a and VviAG1. As such, the aim of this study was to functionally characterise both genes by overexpressing them in tomato and performing phenotypic and gene expression studies. A dual luciferase (DL) assay involving putative target gene promoters was also conducted. Overexpression of VviSOC1a led to the development of leaf-like sepals, petals with increased chlorophyll content and plant sterility phenotypes. VviAG1-OE lines displayed hastened floral initiation, stamenoid petals, dwarfed fruit, as well as forming fleshy fruit sepals which gave the appearance of ripened pericarp tissue. The observed floral phenotypes were, in part, supported by the modulation of genes required for floral organ specification in tomato. VviSOC1a and VviAG1 displayed opposite expression trends, while also repressing each other’s expression in the DL assay. Collectively, the findings of this study supported a role for VviSOC1a in regulating floral organ specification, through the repression of the stamen and carpel identity gene VviAG1. An additional function for VviAG1 in berry development and ripening is also suggested.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Jenna Jolliffe1,2, Claudio Moser2, Stefania Pilati2 and Justin Lashbrooke3*

1South African Grape and Wine Research Institute, Stellenbosch University, Stellenbosch, 7600, South Africa
2Research and Innovation Centre, Edmund Mach Foundation, San Michele all’Adige, 38098, Italy
3Department of Genetics, Stellenbosch University, Stellenbosch, 7600, South Africa

Contact the author*

Keywords

SOC1, AG1, Flower, transcription factor, development

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Red Grenache variety in Rhône Valley : impact of “terroir” and vintages on the aromatic potential of the grapes

The Grenache Noir grape variety, due to its originality and its representativeness, contributes very directly to the quality and typicality of the wines of the Rhône Valley. It is generally appreciated for its varied aromatic palette and for the roundness and suppleness it gives to wines. Since 1995, the Rhodanien Institute has set up a network of reference plots representative of the different types of terroir present in the southern zone of the Côtes du Rhône Appellation (TRUC, 1997; VAUDOUR et al, 1996 ) . Publications on the aromatic composition of grapes and wines are very abundant, but only a few articles have appeared on the Grenache grape variety PAUMES et al., 1986).

Does bioprotection by adding yeasts present antioxydant properties?

AIM: The bioprotection by adding yeasts is an emerging sulfur dioxide alternative. Sulfur dioxide is a chemical adjuvant used for its antiseptic, antioxidasic and antioxidant properties. Faced with the societal demand (Pérès et al., 2018) and considering the proven human risks associated with the total doses of sulfur dioxide (SO2) present in food requirements (García‐Gavín et al., 2012), the reduction of this chemical input is undeniable.

Hanseniaspora in wine-making: their genetic modification and potential role in acid modulation

Hanseniaspora spp. are one of the most common yeast isolates in vineyards and wineries and play an important role in wine-making.

Molecular approaches for understanding and modulating wine taste

Wine consumers generally demand wines having a perception of softer tannins and less ripe, having a heaviness and richness on palate (full-body wine) with a limpid and stable color. However, polyphenol
(tannins)-rich wines have been also correlated with unpleasant taste properties such as astringency and
bitterness when perceived at high intensities. Modulating these unpleasant properties could be important for consumer’s approval of wines.

NEAR INFRARED SPECTROSCOPY FOR THE ESTIMATION OF TEMPRANILLO BLANCO VOLATILE COMPOSITION ALONG GRAPE MATURATION

Grape volatile compounds are mainly responsible for wine aroma, so it is important to know the va-rietal aromatic composition throughout ripening process. Currently, there are no tools that allow mea-suring the aromatic composition of grapes, in intact berries and periodically, throughout ripening, in the vineyard or in the winery. For this reason, this work evaluated the use of near infrared spectroscopy (NIR) to estimate the aromatic composition and total soluble solids (TSS) of Tempranillo Blanco berries during ripening. For this purpose, NIR spectra (1100-2100 nm) were acquired from 240 samples of in-tact berries, collected at different dates, from veraison to overripening.