terclim by ICS banner
IVES 9 IVES Conference Series 9 VviSOC1a and VviAG1 act antagonistically in the regulation of flower formation

VviSOC1a and VviAG1 act antagonistically in the regulation of flower formation

Abstract

The SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1) is a key floral activator that coordinates external and internal stimuli to ensure timely flowering. During early stages of flower formation, SOC1 represses floral organ identity genes such as AGAMOUS (AG) to prevent premature organ differentiation. In addition to floral organ specification, AG has been shown to regulate fleshy fruit expansion and ripening and, as such, is an important contributor to fruit quality traits. Currently, little is known about the function and gene regulatory network of the grapevine homologs VviSOC1a and VviAG1. As such, the aim of this study was to functionally characterise both genes by overexpressing them in tomato and performing phenotypic and gene expression studies. A dual luciferase (DL) assay involving putative target gene promoters was also conducted. Overexpression of VviSOC1a led to the development of leaf-like sepals, petals with increased chlorophyll content and plant sterility phenotypes. VviAG1-OE lines displayed hastened floral initiation, stamenoid petals, dwarfed fruit, as well as forming fleshy fruit sepals which gave the appearance of ripened pericarp tissue. The observed floral phenotypes were, in part, supported by the modulation of genes required for floral organ specification in tomato. VviSOC1a and VviAG1 displayed opposite expression trends, while also repressing each other’s expression in the DL assay. Collectively, the findings of this study supported a role for VviSOC1a in regulating floral organ specification, through the repression of the stamen and carpel identity gene VviAG1. An additional function for VviAG1 in berry development and ripening is also suggested.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Jenna Jolliffe1,2, Claudio Moser2, Stefania Pilati2 and Justin Lashbrooke3*

1South African Grape and Wine Research Institute, Stellenbosch University, Stellenbosch, 7600, South Africa
2Research and Innovation Centre, Edmund Mach Foundation, San Michele all’Adige, 38098, Italy
3Department of Genetics, Stellenbosch University, Stellenbosch, 7600, South Africa

Contact the author*

Keywords

SOC1, AG1, Flower, transcription factor, development

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Development of a new method to understand headspace aroma distribution and explore the pre-sensory level in perceptive interactions involved in red wine fruity aroma expression

A part, at least, of red wines fruity expression may be explained by perceptive interactions involving particularly various substituted ethyl esters and acetates present at concentration far below their olfactory threshold, specifically thanks to synergistic effects. Wine sensory perception is directly linked to the stimulation of the taster at the level of olfactory epithelium by volatiles. These compounds are liberated from the matrix to the atmosphere, and will then be smelt. From a physico-chemical point of view, these volatiles ability to be released may be evaluated by their partition coefficients, which correspond to the volatile concentration ratio between the liquid and gas phase. Our goal is, through these coefficients determination, to assess if volatile matrix composition is able to impact the volatility of some compounds, and then explain sensory perception, i.eto evaluate what is called the pre-sensorial level impact.

Influence of different Lachancea thermotolerans strains in wine acidity

Wine acidity is a parameter of great importance that influences different quality factors of the product such as biological stability or organoleptic characteristics. In the current context of climate change, which gives rise to wines with higher levels of ethanol and lower acidity, the biological acidification with yeast species such as Lachancea thermotolerans could be a solution.
In this work, the effect of the inoculation of different L. thermotolerans on the acidity of wine was studied.

Politics meets terroir. The story of Prosecco – Are GI’s just a protectionist racket?

The recent Free Trade Agreement negotiations between Australia and the European Union have again put the issue of Geographical Indications (GIs) in the spotlight. Australia has long demonstrated its understanding of GIs and maintains a clear and rigorous GI protection system for wine. For many years, Australia’s wine sector was a strong advocate for GIs and a strong system to protect the

History of inorganic and isotopic signatures in Champagne over the last century: lessons

The notion of «terroir» refers to the link between the composition, quality and taste of a wine, on the one hand, and its place of origin, on the other. It involves, among other things, the signature of soil elements, as well as the influence of climatic conditions and plant material used. The composition of the wine is also influenced by the winemaking, storage and bottling processes. We were lucky enough to have a time series of the same champagne, from the end of the first world war to the present. On this exceptional time series, we followed, with the most advanced methods, all the elemental signatures by isotopic multi-dilution, the evolution of the isotopic ratios of heavy elements with very high precision of Sr, Pb, B and Cu.

Comparison of the aroma profile in total and partial dealcoholisation of white and red wines by reverse osmosis

The increasing demand for low-alcohol and non-alcoholic wines has led to the development of advanced dealcoholisation techniques aimed at preserving wine quality while reducing ethanol content. Reverse osmosis is one of the most widely used membrane-based processes for the selective removal of ethanol [1].