terclim by ICS banner
IVES 9 IVES Conference Series 9 VviSOC1a and VviAG1 act antagonistically in the regulation of flower formation

VviSOC1a and VviAG1 act antagonistically in the regulation of flower formation

Abstract

The SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1) is a key floral activator that coordinates external and internal stimuli to ensure timely flowering. During early stages of flower formation, SOC1 represses floral organ identity genes such as AGAMOUS (AG) to prevent premature organ differentiation. In addition to floral organ specification, AG has been shown to regulate fleshy fruit expansion and ripening and, as such, is an important contributor to fruit quality traits. Currently, little is known about the function and gene regulatory network of the grapevine homologs VviSOC1a and VviAG1. As such, the aim of this study was to functionally characterise both genes by overexpressing them in tomato and performing phenotypic and gene expression studies. A dual luciferase (DL) assay involving putative target gene promoters was also conducted. Overexpression of VviSOC1a led to the development of leaf-like sepals, petals with increased chlorophyll content and plant sterility phenotypes. VviAG1-OE lines displayed hastened floral initiation, stamenoid petals, dwarfed fruit, as well as forming fleshy fruit sepals which gave the appearance of ripened pericarp tissue. The observed floral phenotypes were, in part, supported by the modulation of genes required for floral organ specification in tomato. VviSOC1a and VviAG1 displayed opposite expression trends, while also repressing each other’s expression in the DL assay. Collectively, the findings of this study supported a role for VviSOC1a in regulating floral organ specification, through the repression of the stamen and carpel identity gene VviAG1. An additional function for VviAG1 in berry development and ripening is also suggested.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Jenna Jolliffe1,2, Claudio Moser2, Stefania Pilati2 and Justin Lashbrooke3*

1South African Grape and Wine Research Institute, Stellenbosch University, Stellenbosch, 7600, South Africa
2Research and Innovation Centre, Edmund Mach Foundation, San Michele all’Adige, 38098, Italy
3Department of Genetics, Stellenbosch University, Stellenbosch, 7600, South Africa

Contact the author*

Keywords

SOC1, AG1, Flower, transcription factor, development

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Advancement of grape maturity – comparison between contrasting varieties and regions

Grapevine phenology has advanced across many regions, nationally and internationally, in recent decades under the influence of increasing temperatures, resulting in earlier
vintages (Jones and Davis, 2000, Petrie and Sadras, 2008, Tomasi et al., 2011, Webb et al., 2011. Earlier vintages have several ramifications for the wine industry. There are direct implications on quality, due to the fruit ripening during the hotter conditions of summer and early autumn, which then impacts grape composition and wine style (Sadras et al., 2013, Buttrose et al., 1971, Mira de Ordũna, 2010). There are also indirect implications where the fruit is perceived to ripen at a faster rate and the crop reach optimum maturity over a shorter period (Coulter et al., 2016).

Microbial stabilization of wines using innovative coiled UV-C reactor process: impact on chemical and organoleptic proprieties

For several years, numerous studies aimed at limiting the use of SO2 in wines (thermal treatments, pulsed electric fields, microwaves …). Processes must be able to preserve the organoleptic qualities of wines with low energy consumption. In this context, ultraviolet radiations (UV-C), at 254 nm, are well known for their germicidal proprieties. In order to inactivate microorganisms in grape juice and wine without affecting the quality of the product, efficiency of UV-C treatment process should be optimized.

Study of Spanish wine sensory analysis data over a 3-year period

This study presents an investigation based on sensory analysis data of Spanish wines with geographical indications collected over a three-year period. Sensory analysis plays a crucial role in assessing the quality, characteristics, and perception of wines. The trained tasting panel at Dolmar Laboratory, accredited for objective sensory evaluation of wines since 2016, has been tasting over 5000 wines. However, it is since 2021, when a computer application for tastings was developed, that the digitalization of data allows for detailed statistical analysis of the results.

Utilizing ozone for the management of powdery mildew (Erysiphe necator Schwein.) in vineyards: potential and challenges

Powdery mildew, caused by fungal pathogens, poses a significant threat to grapevines in the DOCa Rioja region. In efforts to improve control strategies while reducing reliance on conventional phytosanitary products, ozone could constitute a potential alternative. However, it has short persistence, thus requiring frequent treatments. This study aimed to assess the suitability of ozone as an active substance for controlling powdery mildew within a phytosanitary strategy aimed at reducing conventional phytosanitary product usage. The strategy integrating ozone with conventional products yielded powdery mildew levels comparable to conventional treatments in both disease incidence and severity.

Bioprotective effect of a Torulaspora delbrueckii/Lachancea thermotolerans mixed inoculum and its impact on wines made.

SO2 is an additive widely used as antimicrobial in winemaking industry. However, this compound can negatively affect health, so the search for alternatives is currently a line of research of great interest. One of the proposed alternatives to SO2 as an antimicrobial is the use of bioprotection yeasts, which colonize the medium preventing the proliferation of undesirable microorganisms.