terclim by ICS banner
IVES 9 IVES Conference Series 9 SHIRAZ FLAVONOID EXTRACTABILITY IMPACTED BY HIGH AND EXTREME HIGH TEMPERATURES

SHIRAZ FLAVONOID EXTRACTABILITY IMPACTED BY HIGH AND EXTREME HIGH TEMPERATURES

Abstract

Climate change is leading to an increase in average temperature and in the severity and occurrence of heatwaves, and is already disrupting grapevine phenology. In Australia, with the evolution of the weather of grape growing regions that are already warm and hot, berry composition including flavonoids, for which biosynthesis depends on bunch microclimate, are expected to be impacted [1]. These compounds, such as anthocyanins and tannins, contribute substantially to grape and wine quality. The goal of this research was to determine how flavonoid extraction is impacted when bunches are ex-posed to high (>35 °C) and extreme high (>45 °C) temperatures during berry development and maturity. The sole effect of temperature was investigated on well-irrigated potted Shiraz grapevines grown in a glasshouse, where either the whole vine or bunches-only were heated using fans. For both experiments, berries were sampled at harvest, peeled, ground and total flavonoids were extracted using 60% acetone [2]. Two additional assays evaluated the potential temperature impact on subsequent wine composition using wine-like extraction (15% ethanol) [3] or micro-scale winemaking. Detailed tannin composition was primarily determined by LC-MS/MS after phloroglucinolysis [2], with complementary total tannin concentration (methyl cellulose precipitable assay). Secondary metabolites such as phenolic acid and anthocyanins were also analyzed.

The present work showed that short spells of high temperature may not impact on skin and seed tannin extractability when assessed on visually undamaged berries by harvest. Indeed, while total skin tannin concentrations, extracted with 60% acetone, were clearly reduced by a rise of temperature around véraison, skin extractable tannin (15% ethanol) and seed tannin concentrations were not impacted. In damaged berries at harvest, skin tannins were dramatically reduced while seed tannins were mostly preserved. Wine quality, made with a mix of heat-damaged and undamaged berries, was significantly reduced when about 20% (by mass) of the berries were visually damaged and necrotic, corresponding to about 50% of damaged berries (in number). Maintaining wine quality under a changing climate with more frequent extreme events leading to heat stress and/or water stress is challenging. However, this study showed that the impact of heatwaves in the vineyard may be compensated by a better extraction during winemaking and require further investigations at winery scales.

 

1. Gouot, J. C., Smith, J. P., Holzapfel, B. P., Walker, A. R., & Barril, C. (2019d). Grape berry flavonoids: a review of their biochemical responses to high and extreme high temperatures. Journal of Experimental Botany, 70(2), 397-423
2. Pinasseau, L., Verbaere, A., Roques, M., Meudec, E., Vallverdú-Queralt, A., Terrier, N., Boulet, J.-C., Cheynier, V., & Sommerer, N. (2016). A fast and robust UHPLC-MRM-MS method to characterize and quantify grape skin tannins after chemical depolymerization. Molecules, 21(10), 1409.
3. Bindon, K. A., Kassara, S., & Smith, P. A. (2017). Towards a model of grape tannin extraction under wine-like conditions: the role of suspended mesocarp material and anthocyanin concentration. Australian Journal of Grape and Wine Research, 23(1), 22-32 

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Julia GOUOT1,2, Jason SMITH1,4, Bruno HOLZAPFEL5, Celia BARRIL1,3

1. School of Agricultural, Environmental and Veterinary Sciences, National Wine and Grape Industry Centre, Charles Sturt University, Locked Bag 588, Wagga Wagga, NSW 2678, Australia
2. Current address : Univ. Bordeaux, Bordeaux INP, INRAE, OENO, UMR 1366, ISVV, F-33140 Villenave d’Ornon, France
3. Gulbali Institute, Charles Sturt University, Wagga Wagga, NSW 2678, Australia
4. New South Wales Department of Primary Industries, Orange, New South Wales, 2800, Australia
5. Wagga Wagga Agriculture Institute, New South Wales Department of Primary Industries, Wagga Wagga, NSW 2650, Australia

Contact the author*

Keywords

Extractability, High temperature, Flavonoids, Tannins

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

EFFECT OF MANNOPROTEIN-RICH EXTRACTS FROM WINE LEES ON PHENOLICCOMPOSITION AND COLOUR OF RED WINE

In 2022, wine production was estimated at around 260 million hl. This high production rate implies to generate a large amount of by-products, which include grape pomace, grape stalks and wine lees. It is estimated that processing 100 tons of grapes leads to ~ 22 tons of by-products from which ~ 6 tons are lees [1]. Wine lees are a sludge-looking material mostly made of dead and living yeast cells, yeast debris and other particles that precipitate at the bottom of wine tanks after alcoholic fermentation. Unlike grape pomace or grape stalks, few strategies have been proposed for the recovery and valorisation of wine less [2].

MODULATION OF YEAST-DERIVED AROMA COMPOUNDS IN CHARDONNAY WINES USING ENCAPSULATED DIAMMONIUM PHOSPHATE TO CONTROL NUTRIENT RELEASE

Yeast-derived aroma compounds are the result of different and complex biochemical pathways that mainly occur during alcoholic fermentation. Many of them are related -but not limited- to the availability of nutrients in the fermentation medium and linked to nitrogen metabolism and biomass produced. Besides, the metabolic phase of yeast also regulates the expression of many enzymes involved in the formation of aroma active compounds. The work investigates the overall effect of continuous supplementation of nutrients during alcoholic fermentation of a grape must on the volatile composition of wines.

Rootstock mediated responses of grapevine (Vitis vinifera L.) metabolism and physiology to combined water deficit and salinity stress in Syrah grafts

Water deficit and salinity are increasingly affecting the viticulture and wine industry. These two stresses are intimately related; understanding the physiological and metabolic responses of grapevines to water deficit, salinity and combined stress is critical for developing strategies to mitigate the nega- tive impacts of these stresses on wine grape production. These strategies can include selecting more tolerant grapevine cultivars and graft combinations, improving irrigation management, and using soil amendments to reduce the effects of salinity. For this purpose, understanding the response of grape- vine metabolism to altered water balance and salinity is of pivotal importance.

DISCRIMINATION OF BOTRYTIS CINEREA INFECTED GRAPES USING UNTARGE-TED METABOLOMIC ANALYSIS WITH DIRECT ELECTROSPRAY IONISATION MASS SPECTROMETRY

Infection of grapes (Vitis vinifera) by Botrytis cinerea (grey mould) is a frequent occurrence in vineyards and during prolonged wet and humid conditions can lead to significant detrimental impact on yield and overall quality. Growth of B. cinerea causes oxidisation of phenolic compounds resulting in a loss of colour and formation of a suite of off-flavours and odours in wine made from excessively infected fruit. Apart from wine grapes, developing post-harvest B. cinerea infection in high-value horticultural products during storage, shipment and marketing may cause significant loss in fresh fruits, vegetables and other crops. A rapid and sensitive assessment method to detect, screen and quantify fungal infection would greatly assist viticultural growers and winemakers in determining fruit quality.

VOLATILE AND GLYCOSYLATED MARKERS OF SMOKE IMPACT: LEVELS AND PATTERNS OBSERVED IN 2020 WINES FROM THE UNITED STATES WEST COAST

Smoke impact in wines is caused by a wide range of volatile phenols found in wildfire smoke. These compounds are absorbed and accumulate in berries, where they may also become glycosylated. Both volatile and glycosylated forms eventually end up in wine where they can cause off-flavors, described as “smoky”, “bacon”, “campfire” and “ashtray”, often long-lasting and lingering on the palate. In cases of large wildfire events, economic losses for all wine industry actors can be devastating.