Orals – Oenology, methods of analysis

Non-saccharomyces yeasts gained an increased interest in winemaking during the last decades, due to their ability to produce relevant amounts of polysaccharides. Moreover, a significant release of glutathione into the wine during fermentation was also observed with these strains, as well as an improvement of color stability and wine aroma profile. Valuable results have been obtained by hanseniaspora spp. concerning the release of polysaccharides and the production of acetic esters, mainly during fermentation.

The development of non-animal and non-allergenic alternatives to traditional protein fining agents used in winemaking is of critical importance in order to ensure consumer safety and production sustainability. This study evaluates the effect of protein extracted from three types of algae (spirulina, chlorella vulgaris and tetraselmis chuii) as fining agents on the polymeric proanthocyanidin content responsible for astringency, as well as their effect on the colour, phenolic composition and volatile aroma of two white wines (a and b).

Chitosan is a biopolymer industrially obtained from the deacetylation of chitin, the second most abundant polysaccharide on earth, after cellulose. It is extracted from various terrestrial and marine resources, including insects, grasshoppers, shrimps, crabs, lobsters, squids, and fungi. chitosan has a polycationic character due to the free amine groups along its chemical backbone, and depending on its deacetylation degree (DD) and molecular weight (MW), it shows variable properties that differ from those of other natural polysaccharides.

Bottle ageing is a critical period for wine quality, as it undergoes various chemical and sensory changes during storage. Ideally, a phase of qualitative ageing, during which wine sensory quality improves, is followed by a decline of quality. Understanding how different oenological variables influence these phases is a key challenge in modern winemaking. Recent studies highlighted the significant role of oxygen in modulating reactions involving volatile and non-volatile components, impacting aroma evolution during bottle aging. Oxygen exposure of wine during bottle ageing is mediated by closure.

Despite their significant impact on wine quality and stability, colloids in red wine remain relatively under-researched. A series of studies, developed in the context of the d-wines project, aimed to provide a comprehensive understanding of the structure, composition, and formation mechanisms of red wine colloids by studying monovarietal wines from 10 of the most significant Italian red grape varieties. Starting from the idea that proteins, polysaccharides, and tannins should be involved in colloid formation, 110 monovarietal red wines were analysed for these components, revealing high inter- and intra-varietal diversity [1].

The demand for dealcoholized wine has been progressively increasing in recent years. Moreover, the attention for such products is probably increasing even more. Due to that increasing demand and market awareness the legal authorities are about changing rules for that products. Also, at OIV level, these products are being intensively discussed for certain time. The production of dealcoholized wine bases on wine as initial product. This wine is then reduced by physical methods to an alcohol content of less than 0.5% vol., or in other words, to less than 4g/l of alcohol. There are various technologies are possible for producing dealcoholized wine (Schmitt and Christmann 2019).

Polyphenols are very important compounds of red wines, serving as essential bioactive components and playing an important role in sensory properties. The determination of individual phenolic compounds in red wine is commonly performed by HPLC analysis, while the total polyphenols are quantified by spectrophotometric methods, usually by the method of absorbance at 280 nm (index of ribéreau-gayon) or the method of index of folin-ciocalteu. In this work, we pioneeringly proposed a new and fast method for simultaneous determination of individual and total polyphenols in red wines by direct-injection HPLC without sample preparation.

The european union has estimated that energy consumption for wine production is about 1,750 million kwh per year, of which 500 million kwh is attributable to italy. In recent years, Italy has emerged as the world's leading wine producer with about 50 million hectoliters per year. About 20 percent (9.8 million hectoliters) of Italian wine is marketed after refermentation according to the Charmat-Martinotti method.