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IVES 9 IVES Conference Series 9 Open-GPB 9 Category: Open-GPB-2024 ( Page 5 )

Proceedings of the Open International Conference on Grapevine Physiology and Biotechnology 2024

Discover the abstracts and posters presented during the Open International Conference on Grapevine Physiology and Biotechnology 2024 (Open-GPB2024), held in Logroño, La Rioja, Spain, from July 7th to 11th, 2024. This conference is organised by the Instituto de Ciencias de la Vid y del Vino (ICVV).

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OPEN GPB 2024 – View all

IVES Conference SeriesOpen-GPBOpen-GPB-2024Poster - Advances in omics and big data

Digitalization and valorization of the genotypic and phenotypic information retained within the FEM grapevine germplasm

The maintenance and valorization of genetic diversity is an undoubtable resource for the viticulture of the future, since the climate crisis is forcing us to think of new, more resilient varieties. For this reason, the grapevine germplasm of the Fondazione Edmund Mach has been continuously expanded in the last decade to a total of 3,120 accessions, whose trueness-to-type has been verified by means of the universal set of nine microsatellites. About two thirds are V. vinifera subsp. vinifera accessions, while the rest consists of naturalized and selected hybrids, V. vinifera subsp. sylvestris, and pure species. The genetic material has also been characterized over three consecutive years for ampelographic, vine development, and biotic stress response traits to be exploited for experimental purposes.

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IVES Conference SeriesOpen-GPBOpen-GPB-2024Oral - Abiotic interactions

Diversity of leaf functioning under water deficit in a large grapevine panel: high throughput phenotyping and genetic analyses

Water resource is a major limiting factor impacted by climate change that threatens grapevine production and quality. Understanding the ecophysiological mechanisms involved in the response to water deficit is crucial to select new varieties more drought tolerant. A major bottleneck that hampers such advances is the lack of methods for measuring fine functioning traits on thousands of plants as required for genetic analyses. This study aimed at investigating how water deficit affects the trade-off between carbon gains and water losses in a large panel representative of the Vitis vinifera genetic diversity. 250 genotypes were grown under 3 watering scenarios (well-watered, moderate and severe water deficit) in a high-throughput phenotyping platform.

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IVES Conference SeriesOpen-GPBOpen-GPB-2024Oral - New biotechnological tools

DNA-free editing to improve stress resilience of wine grape genotypes recalcitrant-to-regeneration

Wine viticulture, being firmly linked to the vine-terroir relationship, has always encountered significant bottlenecks to genetic innovation. Nonetheless, the development of new breeding strategies leading to the selection of stress resilient genotypes is urgent, especially in viticulture, where it would allow reducing the use of chemical treatments adopted to control fungal diseases. Genome editing represents an extremely promising breeding technique. Unfortunately, the well-known recalcitrance of several wine grape cultivars to in vitro regeneration strongly limits the exploitation of this approach, which to our knowledge has so far been developed on table grape genotypes with high regeneration potential.

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IVES Conference SeriesOpen-GPBOpen-GPB-2024Oral - New biotechnological tools

DNA-Free genome editing confers disease resistance in grapevine

Grapevine (Vitis Vinifera L.), one of the most important cultivated fruit crops, is facing significant challenges due to climate change. Specifically, increasing temperatures negatively impact the physiological traits and disrupt plant phenology. Additionally, increased virulence in pathogen attacks and pests leads to significant yield loss, requiring widespread application of plant protection products. Traditional agronomic practices offer only partial mitigation, requiring the development of precise and effective intervention strategies. The economic worth of viticulture has prompted continuous efforts in grapevine genetic improvement programs, traditionally involving conventional breeding and clonal selection that, however, are complex and time-consuming approaches.

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IVES Conference SeriesOpen-GPBOpen-GPB-2024Poster - Plant vegetative and reproductive development

Does foliar fertilization with Seaweed improve the productivity and quality of ‘Merlot’ grape must?

Developing technologies that help vines survive and produce in quantity and quality within current times is mandatory. In this sense, in the 2021/2022 agricultural harvest, the influence of the foliar application of seaweed - Laminaria japonica was studied, aiming at productivity and quality of the must in the ‘Merlot’ grape. In the city of “Santana do Livramento”, “Rio Grande do Sul” (RS), Brazil; in a 15-year-old commercial vineyard of ‘Merlot’ clone ENTAV-INRA® 347, grafted onto ‘SO4’ rootstock, the following treatments were applied on 6 occasions: No treatment (control) and; Foliar application of Laminaria japonica seaweed (commercial product: Exal (ALAS), 2 kg ha-1).

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IVES Conference SeriesOpen-GPBOpen-GPB-2024Poster - Plant vegetative and reproductive development

Dormancy conundrum: thermal requirements plasticity to reach budburst may be explained by annual environmental dynamics

Deciphering grapevine dormancy is crucial in the current context of climatic challenges: advancing budburst phenology and increased late frost probabilities, observed in the last decades and expected to further increase, require deeper understanding. Beyond higher mean temperatures, abiotic stresses such as water deficit have also been emphasized as actors. In this framework, we aimed at exploring new methodologies for tracking dormancy cycle and testing the interplay on its regulation of temperature dynamics and drought.
In a first experiment, twenty-one Vitis vinifera varieties were monitored during ecodormancy and budburst over three years.

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Flash - Abiotic interactionsIVES Conference SeriesOpen-GPBOpen-GPB-2024

Drought affects vineyard soil microbiome: approach to select micro-organisms adapted to drought

Climate transition with frequent heat waves and long drought periods threatens grapevine productivity and wine quality in the Mediterranean regions. Microorganisms are known to contribute to plant fitness and to stimulate plant resilience against biotic and abiotic factors.
In this work, it was assessed the impact of long-term drought on soil microbiome associated to grapevine in open field in Alentejo, renowned Portuguese wine region.
Soil and plant tissues of drought tolerant Syrah cultivar exposed to three irrigation levels (100%- FI, 50%-DI ETc; rain-fed–NI) for 5 years were sampled for two years (2022-2023). Metabarcoding analysis of soil bacteria (16S V4 rRNA) and fungi (ITS sub-region) were integrated with soil physiochemical properties and leaves´ physiological data. Pre-dawn leaf water potential and stomatal conductance confirmed the imposed drought scenarios. Even though, α- and β-diversity of prokaryotic and eukaryotic microbial communities differed more by season than water availability, samples clustered according to soil water content and pH (p<0.05). Fungal communities show higher differences in the structure across treatments than bacteria. In 2023, 16 bacterial against 61 fungal ASVs were significatively different in abundance between NI and FI. Beijerinckiaceae, Bradyrhizobiaceae (Alphaproteobacteria) and Nocardioidaceae, Streptomycetaceae (Actinobacteria) families resulted to be significatively more abundant in NI, while Ascomycota, Basidyomicota and Mortierellomycota are the most important fungal phyla in NI. With culturomics data, this study aims to gather insights into how soil microbiome is remodelled under drought and contribute to select bacterial and fungal taxa with potential to mitigate drought stress in vineyards.

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IVES Conference SeriesOpen-GPBOpen-GPB-2024Poster - Abiotic interactions

Drought responses in Chardonnay and Sauvignon blanc grapevine cultivars: Mechanistic insights and varietal contrasts

This study explored the responses of Chardonnay and Sauvignon blanc grapevine cultivars to water deficit across four years, uncovering their shared patterns and distinctive coping mechanisms. The research was conducted in a commercial vineyard located in Isla de Maipo, Chile. Various characterization approaches were employed including plant water potentials (), gas exchange measurements, shoot vulnerability curves, productivity assessments, and leaf cell water relations. Linear mixed models and sensitivity analyses were performed using various statistical methods to evaluate cultivar responses to water deficit. As the water deficit progressed, both cultivars displayed a parallel reduction in stomatal conductance, leaf turgor, and increased shoot embolism.

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IVES Conference SeriesOpen-GPBOpen-GPB-2024Oral - Advances in precision viticulture and phenotyping

Dry leaf hyperspectral reflectance predicts leaf elemental composition in grafted hybrids

Elemental composition, measured as the concentrations of different elements present in a given tissue at a given time point, is a key indicator of vine health and development. While elemental composition and other high-throughput phenotyping approaches yield tremendous insight into the growth, physiology, and health of vines, costs and labor associated with repeated measures over time can be cost-prohibitive. Recent advances in handheld sensors that measure hyperspectral reflectance patterns of leaf tissue may serve as an affordable proxy for other types of phenotypic data, including elemental composition. Here, we ask if reflectance patterns of dried Chambourcin leaf tissue from an experimental grafting vineyard can predict the known elemental composition of those leaves.

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IVES Conference SeriesOpen-GPBOpen-GPB-2024Poster - Abiotic interactions

Effect of different canopy managements on microclimate and carbon allocation in Vitis vinifera cv Chardonnay

Climate change strongly affects the wine-growing sector which increasingly requires in situ adaptation strategies aimed at preserving the sustainability of production. Investigating microclimate becomes crucial in comprehending environmental pressures on plants. The microclimatic investigation conducted in the Orvieto PDO (central Italy) allowed us to highlight the climatic dynamics occurring in the last 25 years and the frequency and intensity of abiotic stresses. Two management strategies for the canopy were identified: early defoliation (ELR) and foliar application of Basalt Flour ® (FB) compared to the ordinary management (C) of the company (bud selection and topping). The effects on plant vigour indices (LAI), resource allocation in terms of carbon stored in the above-ground organs of the vine, and the microclimate of the canopy and the berry were evaluated. In particular, microclimate was evaluated through a network of sensors connected wirelessly (Wireless Sensor Network), dedicated to collecting information on temperature and humidity in the canopy and clusters.

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