Typically, subjective, and visual methods are used by grape growers to assess harvest maturity. These methods may not accurately represent the maturity of an entire vineyard – especially if extensive and representative sampling was not used. New technologies have been investigated for improved harvest management decisions. Spectroscopy methods utilizing the near-infrared region of the light spectrum is one such technology investigated as an alternative to classic methods and particularly the application of hyperspectral imaging (HSI) has recently gained attention in research. HIS is a spectroscopic technique that obtains hundreds of images at different wavelengths collecting spectral data for each pixel in the sample i.e., providing both spectral and spatial data.
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NIR based sensometric approach for consumer preference evaluation
Climate change has had a global impact on grape production, and as a result, developing table grape varieties that can withstand climate-related threats has become a significant goal. However, it is equally important to ensure that these new grape varieties meet the preferences of consumers. To achieve this goal, a procedure has been developed that combines sensory analysis with spectroscopic data collected in the NIR region. Each sample was analyzed using both traditional analytical techniques and non-destructive NIR spectroscopy.
Withering of the ‘Moscato giallo’ grapes under covered space
For the purpose of producing predicate wines in northern part of Croatia, grapes are traditionally left on the vine unpicked. However, grapes on the vine are exposed to unfavorable environmental conditions that affect rapid rotting and attacked by birds. To eliminate the mentioned risks, the grapes can be picked and placed in a protected space (loft, greenhouse, etc.) suitable for drying. This study presents the results of research on withering grapes of the ‘Moscato giallo’ variety in two tretment: sun drying (under covered terrace) and drying in the shade (loft). The following quality parameters were monitored: mass of grapes, sugar concentration, content of total acids, pH, content of organic acids.
Volatile Organic Compound markers of Botrytis cinerea infection in artificially inoculated intact grape berries
The addition of partially dehydrated grapes to enrich must composition for producing complex dry/sweet wines represents a traditional practice in several regions of the world. However, the environmental conditions of dehydration chambers may facilitate the infection of Botrytis cinerea Pers. by promoting disease and provoking large grape losses. B. cinerea attack can induce alterations in the profile of volatile organic compounds (VOCs), which could be detected by sensors specifically trained to detect infection/disease-related compounds. These sensors could facilitate the early detection of the infection, consequently allowing to adjust some dehydration parameters.
New biotechnological approaches for a comprehensive characterization of AGL11 and its molecular mechanism underlying seedlessness trait in table grape
In table grapes seedlessness is a crucial breeding target, mainly results from stenospermocarpy, linked to the Thompson Seedless variety. Several studies investigated the genetic control of seedlessness identifying AGL11, a MADS-box transcription factor, as a crucial gene.
We performed a deep investigation of the whole AGL11 gene sequence in a collection of grapevine varieties revealing three different promoter-CDS combinations. By investigating the expression of the three AGL11 alleles and evaluating their ability to activate the promoter region, we show that AGL11 regulates its transcription in a specific promoter-CDS manner. By a multi-AGL11 co-expression analysis we identified a methyl jasmonate esterase, an indole-3-acetate beta-glucosyltransferase, and an isoflavone reductase as top AGL11 candidate targets. In vivo experiments further confirmed AGL11 role in regulating these genes, demonstrating its significant influence in seed development and thus in seedlessness trait.
Melatonin priming retards fungal decay in postharvest table grapes
Postharvest losses of fruits may reach in some cases 40% in developed countries. This food waste has a significant carbon footprint and makes a major contribution toward greenhouse gas emissions so sustainable postharvest strategies are being investigated.
Melatonin, a well-known mammalian neurohormone, has been investigated as a priming agent to slow down fungal decay progression in postharvest climacteric and some non-climacteric fruits. However, the molecular and metabolic mechanisms responsible for such enhancement of disease tolerance are largely unknown.
Screening table grape cultivars using cell wall ELISA and glycan microarrays for berry firmness and quality parameters
The crunchy texture of table grapes is one of the key quality parameters during production. This varies from cultivar to cultivar, stage of harvest and vineyard performance. Cell wall properties are key drivers of berry quality (e.g., pericarp firmness and intactness) at harvest and beyond. Common practise amongst producers is to continuously monitor firmness by evaluating pericarp appearance of cross-sectioned berries prior to harvest. These qualitative methods can be quite arbitrary and imprecise in their execution, but more quantitative, yet simple and high-throughput methods to evaluate these cell wall polymers are not yet readily available.
Application of Hyper Spectral Imaging for early detection of rachis browning in table grapes
Rachis browning is a common abiotic stress that occurs during postharvest storage, leading to a decrease in commercial value of table grapes and resulting in significant economic losses. Its early detection could enable the implementation of preventive strategies. In this report, we show the feasibility of a non-destructive early detection of browning based on Hyper Spectral Imaging (HSI). Furthermore, rachis samples were subjected to transcriptomic analysis to understand putative pathways causing differences in browning within varieties.
Enhancing table grape production: addressing challenges and opportunities for sustainability and quality improvement
Table grapes, being consumed as fresh, raisins, and transformed products are among the most appreciated fruits worldwide. Its popularity is increasing also due to its organoleptic and nutritional qualities that meet the consumers’ interest in healthier foods. Recent data from International Organization of Vine and Wine (OIV) revealed that table grape production has doubled in the last twenty years, and varietal availability has increased thanks to the several breeding programs.
To maintain the socio-economic impact of this sector, new challenges need to be addressed.
Innovative approaches for fungicide resistance monitoring in precision management of grapevine downy mildew
Effective control with fungicides is essential to protect grapevine from downy mildew, a devastating disease caused by the oomycete Plasmopara viticola. Managing this disease faces challenges in maintaining fungicide efficacy as the number of modes of action decreases and the risk of fungicide resistance increases. Long-term measures should address strains resistant to multiple modes of action, that can be selected by the repeated use of single-site fungicides. For these reasons, a precision management of the disease, that considers the selection of the best fungicide schedule according to the sensitivity profile of the pathogen population, is needed.
Oospore germination dynamics and disease forecasting model for a precision management of downy mildew
Downy mildew, caused by Plasmopara viticola, is the most economically impactful disease affecting grapevines. This polycyclic pathogen triggers both primary and secondary infection cycles, resulting in significant yield losses when effective disease control measures are lacking. Over the winter, the pathogen survives by forming resting structures, the oospores, derived from sexual reproduction, which produce the inoculum for primary infections. To optimize grapevine downy mildew control and obtain the desired levels of production while minimizing chemical inputs, it is crucial to optimize the timeframe for fungicide application. Disease forecasting models are useful to identify the infection risk.
Investigating water stress-related seasonal and spatial patterns and the possible links with juice and wine compositional parameters
The mapping of spatial variability in vineyards offers the potential to implement zonal management strategies with the aim to optimize economic benefits and increase sustainability by managing natural resources, such as water used for irrigation, more optimally. This study characterized the (natural) variability in plant water status in a commercial Cabernet Sauvignon block, using remote sensing techniques, and identified the impact of this variability on the yield, and juice and wine composition. From the field data collected over two growing seasons, we demonstrated that remote sensing techniques are a practical and powerful tool for mapping spatial variability within vineyard blocks.
Exploring high throughput secondary trait phenomics to improve grapevine breeding
Modern grapevine breeding programs have overcome many challenges using genomic selection, which has allowed breeders to make targeted selections at earlier stages in the breeding process. However, the cost of genetic testing may present a burden for some programs, and markers often struggle to accurately predict quantitative traits. Recent advances in high throughput, high-dimensional data have provoked investigation into the use of high-dimensional phenomics as a low-cost addition to the grape breeder’s toolkit that may offer advantages in predicting quantitative traits. High-dimensional secondary trait (HDST) data has been employed in annual crops for prediction of agriculturally important traits such as yield.
A novel dataset and deep learning object detection benchmark for grapevine pest surveillance
Flavescence dorée (FD) stands out as a significant grapevine disease with severe implications for vineyards. The American grapevine leafhopper (Scaphoideus titanus) serves as the primary vector, transmitting the pathogen that causes yield losses and elevated costs linked to uprooting and replanting. Another potential vector of FD is the mosaic leafhopper, Orientus ishidae, commonly found in agroecosystems. The current monitoring approach involves periodic human identification of chromotropic traps, a labor-intensive and time-consuming process.
Application of satellite-derived vegetation indices for frost damage detection in grapevines
Wine grape production is increasingly vulnerable to freeze damage due to warming climates, milder winters, and unpredictable late spring frosts. Traditional methods for assessing frost damage in grapevines which combine fieldwork and meteorological data, are expensive, time-consuming, and labor-intensive. Remote sensing could offer a rapid, inexpensive way to detect frost damage at a regional scale. Remote sensing approaches were used to assess freeze damage in grapevines by evaluating satellite-derived vegetation indices (VIs) to understand the severity and spatial distribution of damage in several New York vineyards immediately after a frost event (May 17th-18th, 2023). PlanetScope 3m satellite images acquired before and after the freeze were used to map damage and measure changes in VIs for vineyards in the Finger Lakes region.
Protection of genetic diversity: maintenance and developements of a grapevine genebank in Hungary
Among the items preserved in gene banks, the old standard and autochthonous varieties represent an increasing value, since these varieties may have properties to make their cultivation more effective under changing climatic conditions. The increasingly extreme weather is a huge challenge for the viticulture. Collectional varieties can also play important role in protection against pests and pathogens. A genebank ensures not only the preservation of rare varieties, but also gives the opportunity for more knowledge and research of these varieties.
Learning from remote sensing data: a case study in the Trentino region
Recent developments in satellite technology have yielded a substantial volume of data, providing a foundation for various machine learning approaches. These applications, utilizing extensive datasets, offer valuable insights into Earth’s conditions. Examples include climate change analysis, risk and damage assessment, water quality evaluation, and crop monitoring. Our study focuses on exploiting satellite thermal and multispectral imaging, and vegetation indexes, such as NDVI, in conjunction with ground truth information about soil type, land usage (forest, urban, crop cultivation), and irrigation water sources in the Trentino region in North-East of Italy.
High throughput winter pruning weight estimation based on wood volume evaluation
There is currently a real need to improve and speed-up phenotyping in experimental set-ups to increase the number of modalities studied. Accurate information acquisition on plant status with high-throughput capacity is the main appeal of on-board systems.
A proximal sensing camera for a proxy of winter pruning weight was tested. We estimated the shoot volume of the vine by image analysis using algorithms that integrate the local shoot section area estimate along the shoot skeleton obtained by a morphological distance transform.
The study was carried out on the GreffAdapt experimental vineyard in Guyot simple training and a canopy management using vertical trellising. The planting density is 6250 vines/ha with a row spacing of 1.6×1m. Five scions grafted onto 55 rootstocks are present and the combination rootstock×scion is different every five plants.