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IVES 9 IVES Conference Series 9 Open-GPB 9 Category: Open-GPB-2024 ( Page 2 )

Proceedings of the Open International Conference on Grapevine Physiology and Biotechnology 2024

Discover the abstracts and posters presented during the Open International Conference on Grapevine Physiology and Biotechnology 2024 (Open-GPB2024), held in Logroño, La Rioja, Spain, from July 7th to 11th, 2024. This conference is organised by the Instituto de Ciencias de la Vid y del Vino (ICVV).

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OPEN GPB 2024 – View all

IVES Conference SeriesOpen-GPBOpen-GPB-2024Oral - Table grapes, raisins and postharvest physiology

Application of Hyper Spectral Imaging for early detection of rachis browning in table grapes

Rachis browning is a common abiotic stress that occurs during postharvest storage, leading to a decrease in commercial value of table grapes and resulting in significant economic losses. Its early detection could enable the implementation of preventive strategies. In this report, we show the feasibility of a non-destructive early detection of browning based on Hyper Spectral Imaging (HSI). Furthermore, rachis samples were subjected to transcriptomic analysis to understand putative pathways causing differences in browning within varieties.

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IVES Conference SeriesOpen-GPBOpen-GPB-2024Poster - Fruit and wine yield and composition

Application of nitrogen forms such as nitrate, urea, and amino acids effects on leaf and berry physiology and wine quality

Nitrogen (N) uptake by grapevine roots in forms like nitrate, ammonium, urea, or amino acids influences vegetative and generative growth, impacting grape quality and wine sensory profile. The study examined nitrogen's influence on phenolic compounds in leaves, berries, and wine across different scales — hydroponics, soil culture, and vineyard trials. Nitrogen forms altered metabolite patterns in leaves and wine significantly, affecting aroma and flavor. Key nitrogen assimilation enzymes (NR, NiR, GS) in grapevine rootstocks responded to nitrogen forms and timing. Hydroponically grown rootstocks fertilized with various forms showed differences in enzyme expression and activity, suggesting rootstocks can assimilate amino acid glutamine (Gln).

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IVES Conference SeriesOpen-GPBOpen-GPB-2024Poster - Advances in precision viticulture and phenotyping

Application of satellite-derived vegetation indices for frost damage detection in grapevines

Wine grape production is increasingly vulnerable to freeze damage due to warming climates, milder winters, and unpredictable late spring frosts. Traditional methods for assessing frost damage in grapevines which combine fieldwork and meteorological data, are expensive, time-consuming, and labor-intensive. Remote sensing could offer a rapid, inexpensive way to detect frost damage at a regional scale. Remote sensing approaches were used to assess freeze damage in grapevines by evaluating satellite-derived vegetation indices (VIs) to understand the severity and spatial distribution of damage in several New York vineyards immediately after a frost event (May 17th-18th, 2023). PlanetScope 3m satellite images acquired before and after the freeze were used to map damage and measure changes in VIs for vineyards in the Finger Lakes region.

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Flash - Abiotic interactionsIVES Conference SeriesOpen-GPBOpen-GPB-2024

Arinto clones tolerant to climate change: in depth transcriptomic study of tolerant and sensitive genotypes

Drought and heat waves deriving from climate change have been affecting grapevine plants and altering wine characteristics in the past years, and effects are expected to get worst. Innovative approaches to address this problem have been undertaken in several varieties, that consist in exploring intravarietal variability to identify genotypes that are tolerant to abiotic stress. Such is the case of the variety Arinto, where an experimental population of 165 clones installed according to a resolvable row-column design with 6 replicates, was scanned for several parameters, including surface leaf temperature (SLT). Linear mixed models were fitted to the data of the traits evaluated, and the empirical best linear unbiased predictors (EBLUPs) of genotypic effects for each trait were obtained as well as the coefficient of genotypic variation (CVG) and broad sense heritability.

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IVES Conference SeriesOpen-GPBOpen-GPB-2024Poster - Advances in omics and big data

Artificial intelligence (AI)-based protein modeling for the interpretation of grapevine genetic variants

Genetic variants known to produce single residue missense mutations have been associated with phenotypic traits of commercial interest in grapevine. This is the case of the K284N substitution in VviDXS1 associated with muscat aroma, or the R197L in VviAGL11 causing stenospermocarpic seedless grapes. The impact of such mutations on protein structure, stability, dynamics, interactions, or functional mechanism can be studied by computational methods, including our pyDock scoring, previously developed. For this, knowledge on the 3D structure of the protein and its complexes with other proteins and biomolecules is required, but such knowledge is not available for virtually none of the proteins and complexes in grapevine.

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IVES Conference SeriesOpen-GPBOpen-GPB-2024Poster - Fruit and wine yield and composition

Autochthonous non-Saccharomyces extra-cellular metabolism of tryptophan, tyrosine, and phenylalanine

Amino acids are crucial nitrogen sources in yeast metabolism, influencing both biomass production and fermentation rate. The breakdown byproducts of amino acids contribute to the aroma of the wine and wine's health benefit compounds. This study focused on the yeast's extracellular metabolic profile of tryptophan, tyrosine, and phenylalanine belonging to the group of aromatic amino acids in experimental Maraština wines. Alcoholic fermentations were conducted on sterile grape Maraština must using seven autochthonous non-Saccharomyces yeasts in sequential fermentation with commercial Saccharomyces cerevisiae.

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IVES Conference SeriesOpen-GPBOpen-GPB-2024Oral - Fruit and wine yield and composition

Berry shrivel causes – summarizing current hypotheses

Diverse ripening disorders affect grapevine resulting in high economic losses worldwide. The common obvious symptom is shriveling berries, however the shriveling pattern and the consequences for berry quality traits are distinct in each disorder. Among them, the disorder berry shrivel is characterized by a reduced sugar accumulation short after the onset of berry ripening leaving the clusters unsuitable for wine processing. Although our knowledge on BS increased recently, potential internal or external triggers contributing to the induction of BS are yet to be explored.

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IVES Conference SeriesOpen-GPBOpen-GPB-2024Oral - Biotic interactions

Biotic interactions: case of grapevine cultivars – the fungal pathogen Neofusicoccum parvum – biocontrol agents 

Grapevine is subject to multiple stresses, either biotic or abiotic, frequently in combination. These stresses may negatively impact the health status of plants and reduce yields. For biotic stress, grapevine is affected by numerous pest and diseases such as downy and powdery mildews, grey mold, black rot, grapevine fanleaf virus and trunk diseases (namely GTDs). The interaction between grapevine and pathogens is relatively complex and linked to various pathogenicity factors including cell-wall-degrading enzymes (especially CAZymes) and phytotoxic secondary metabolites, growth regulators, effectors proteins, and fungal viruses.

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IVES Conference SeriesOpen-GPBOpen-GPB-2024Oral - Biotic interactions

Cell wall remodeling mediated by specific PME genes plays a role in grapevine response to Botrytis cinerea

Botrytis cinerea (Bc) is one of the main pathogens affecting the cultivated grapevine. A key role in grapevine tissue colonization is played by cell wall (CW) remodeling driven by CW Modifying Enzymes (CWMEs), expressed both by the host and the pathogen. Their action can impact CW integrity and trigger specific immune signaling, thus influencing Bc infection outcome. To further characterize the role of the CW in the grapevine response to Bc, two contrasting genotypes in their resistance to the fungus were artificially inoculated at full bloom. RNA-seq analysis and biochemical characterization of the CW and its modification in samples collected at 24 hours post-inoculation highlighted significant differences between genotypes.

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IVES Conference SeriesOpen-GPBOpen-GPB-2024Poster - Biotic interactions

Challenges for the Implementation of commercial inoculum of arbuscular fungi in a commercial Callet vineyard (Vitis vinifera L.)

Over the past 70 years, scientific literature has consistently illustrated the advantageous effects of arbuscular mycorrhiza fungi (AMF) on plant growth and stress tolerance. Recent reviews not only reaffirm these findings but also underscore the pivotal role of AMF in ensuring the sustainability of viticulture. In fact, various companies actively promote commercial inoculants based on AMF as biofertilizers or biostimulants for sustainable viticulture. However, despite the touted benefits of these products, the consistent effectiveness of AMF inoculants in real-world field conditions remains uncertain.

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